Naslov
Matrix Solid-Phase Dispersion Extraction and Gas Chromatographic Determination of Chloramphenicol in Muscle Tissue

Autori
Kubala-Drinčić, Helena ; Bažulić, Davorin ; Sapunar-Postružnik, Jasenka ; Grubelić, Mirela ; Stuhne, Goran

Izvornik
Journal of agricultural and food chemistry (0021-8561) 51 (2003), 4; 871-875

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
chloramphenicol; gas chromatography; matrix solid-phase dispersion; muscle tissue; residues

Sažetak
A method based on matrix solid-phase dispersion (MSPD) was developed for the gas chromatographic (GC) determination of chloramphenicol (CAP) residues in animal muscle tissue. Muscle tissue was blended with octadecylsilyl derivatized silica (C18). A column made from C18/muscle tissue matrix was washed with n-hexane and acetonitrile-water (5+95), after which CAP was eluted with acetonitrile-water (50+50) and partitioned into ethyl acetate. The final extract was evaporated and a trimethylsilyl derivative of CAP was prepared with Sylon HTP and detected by GC with an electron capture detector (ECD) and a mass spectrometer. For quantitation, the internal standard used was the meta isomer of CAP (m-CAP) for GC-ECD. Muscle tissue samples were fortified at three concentration levels. At 5, 10 and 15 microg/kg levels the respective mean recoveries were 93, 96 and 98%, and the repeatabilities were 13, 11 and 3%. The detection and quantitation limits with ECD were 1.6 and 4.0 microg/kg, respectively. No statistically significant difference was observed in the efficiency of CAP extraction from muscle tissue of various animals (bovine, porcine and poultry) by the MSPD technique.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

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