Naslov
Pitfalls in PAP stain with Xylene Substitute

Autori
Bauer Šoštarić, Darija ; Ranogajec, Irena ; Moslavac, Sandra

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Journal of Cytopathology Abstracts of the 37th ERuropean Congress of Cytopathology / Herbert, Amanda - : Wiley-Blackwell, 2012, 96-96

Skup
37th European Congress of Cytology

Mjesto i datum
Cavtat, Hrvatska; Dubrovnik, Hrvatska, 30.09.2012. - 03.10.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
PAP stain ; PAP smear ; Xylene substitute

Sažetak
Background: Papanicolaou stain (PAP stain) is a multichromatic staining cytological technique. PAP stain is used to differentiate cells in gynaecological smears (PAP smears) and smear preparations of various bodily secretions-sputum, brushing, washings, urine, fine needle aspiration materials, fluids or other materials containing cells. There are four main steps on the staining procedure: fixation, nuclear staining, cytoplasmic staining and clearing. CASE: Five years ago, we started using xylene substitute for clearing PAP smears and the r4esults were unsatisfactory-the nuclei were pale, cytoplasmic color was not consistent, the part of smear was yellow, gray or pink with no possibility to detect and categorize cellular abnormalities, The stains were used for appropriate staining times, changed at the proper intervals, and used in conjunction with suitable rinses that were also changed at the proper intervals. After checking the specifications of alcohols produced by two different manufacturers, we discovered that although the notes on the labels were the same, the broader specifications revealed differences between purity and volatile impurities values. Staining procedure with high quality alcohol showed much better results-the cells were clearly visible at low power (10x objectives) and blue to purple in color. At high power (40x objectives) nuclear chromatin was clearly demonstrated and appeared granular, crisp and distinct, so the Pap smear could had been interpreted in the best way. Conclusion: Our experience allows us to conclude that the best results in staining procedure with xylene substitute are achieved by using alcohol with Ph.Eur specification

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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