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Pregled bibliografske jedinice broj: 1182383

The roles of diol dehydratase from pdu operon on glycerol catabolism in Klebsiella pneumoniae

Shu, Lin; Wang, Qinghui; Jiang, Weiyan; Tišma, Marina; Oh, Beakrock; Shi, Beakrock; Lye, Gary J.; Baganz, Frank; Wei, Dong; Hao, Jian
The roles of diol dehydratase from pdu operon on glycerol catabolism in Klebsiella pneumoniae // Enzyme and microbial technology, 157 (2022), 110021, 8 doi:.org/10.1016/j.enzmictec.2022.110021 (međunarodna recenzija, članak, znanstveni)

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Naslov
The roles of diol dehydratase from pdu operon on glycerol catabolism in Klebsiella pneumoniae

Autori
Shu, Lin ; Wang, Qinghui ; Jiang, Weiyan ; Tišma, Marina ; Oh, Beakrock ; Shi, Beakrock ; Lye, Gary J. ; Baganz, Frank ; Wei, Dong ; Hao, Jian

Izvornik
Enzyme and microbial technology (0141-0229) 157 (2022); 110021, 8

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Glycerol dehydratase ; Diol dehydratase ; Microcompartment ; 1, 3-propanediol ; Klebsiella pneumoniae

Sažetak
The dha operon of Klebsiella pneumoniae is responsible for glycerol catabolism and 1, 3-propanediol formation. Subunits of glycerol dehydratase and the large subunit of glycerol dehydratase reactivating factor are encoded by dhaBCE and dhaF, respectively. Proteins of pdu operon form a microcompartment (bacteria organelle) and responsible for 1, 2-propanediol catabolism. In this operon, pduCDE and pduG encode subunits of diol dehydratase and its reactivating factor. Diol dehydratase is an isofunctional enzyme of glycerol dehydratase, but its role in glycerol catabolism was not entirely clear. In this study, dhaBCE, pduCDE, dhaF, and pduG in K. pneumoniae were knocked out individually or combinedly. These strains were cultured with glycerol as a substrate, and dehydratase activities in the cytoplasm and microcompartment were detected. Results showed that glycerol dehydratase and diol dehydratase were simultaneously responsible for glycerol catabolism in K. pneumoniae. Besides being packaged in microcompartment, large amounts of diol dehydratase was also presented in the cytoplasm. However, the Pdu microcompartment reduced the accumulation of 3-hydroxypropionaldehyde in the fermentation broth. PduG can cross reactivate glycerol dehydratase instead of DhaF. However, DhaF is not involved in reactivation of diol dehydratase. In conclusion, diol dehydratase and Pdu microcompartment play important roles in glycerol catabolism in K. pneumoniae.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA

Profili:

Avatar Url Marina Tišma (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com

Citiraj ovu publikaciju:

Shu, Lin; Wang, Qinghui; Jiang, Weiyan; Tišma, Marina; Oh, Beakrock; Shi, Beakrock; Lye, Gary J.; Baganz, Frank; Wei, Dong; Hao, Jian
The roles of diol dehydratase from pdu operon on glycerol catabolism in Klebsiella pneumoniae // Enzyme and microbial technology, 157 (2022), 110021, 8 doi:.org/10.1016/j.enzmictec.2022.110021 (međunarodna recenzija, članak, znanstveni)
Shu, L., Wang, Q., Jiang, W., Tišma, M., Oh, B., Shi, B., Lye, G., Baganz, F., Wei, D. & Hao, J. (2022) The roles of diol dehydratase from pdu operon on glycerol catabolism in Klebsiella pneumoniae. Enzyme and microbial technology, 157, 110021, 8 doi:.org/10.1016/j.enzmictec.2022.110021.
@article{article, author = {Shu, Lin and Wang, Qinghui and Jiang, Weiyan and Ti\v{s}ma, Marina and Oh, Beakrock and Shi, Beakrock and Lye, Gary J. and Baganz, Frank and Wei, Dong and Hao, Jian}, year = {2022}, pages = {8}, DOI = {doi.org/10.1016/j.enzmictec.2022.110021}, chapter = {110021}, keywords = {Glycerol dehydratase, Diol dehydratase, Microcompartment, 1, 3-propanediol, Klebsiella pneumoniae}, journal = {Enzyme and microbial technology}, doi = {doi.org/10.1016/j.enzmictec.2022.110021}, volume = {157}, issn = {0141-0229}, title = {The roles of diol dehydratase from pdu operon on glycerol catabolism in Klebsiella pneumoniae}, keyword = {Glycerol dehydratase, Diol dehydratase, Microcompartment, 1, 3-propanediol, Klebsiella pneumoniae}, chapternumber = {110021} }
@article{article, author = {Shu, Lin and Wang, Qinghui and Jiang, Weiyan and Ti\v{s}ma, Marina and Oh, Beakrock and Shi, Beakrock and Lye, Gary J. and Baganz, Frank and Wei, Dong and Hao, Jian}, year = {2022}, pages = {8}, DOI = {doi.org/10.1016/j.enzmictec.2022.110021}, chapter = {110021}, keywords = {Glycerol dehydratase, Diol dehydratase, Microcompartment, 1, 3-propanediol, Klebsiella pneumoniae}, journal = {Enzyme and microbial technology}, doi = {doi.org/10.1016/j.enzmictec.2022.110021}, volume = {157}, issn = {0141-0229}, title = {The roles of diol dehydratase from pdu operon on glycerol catabolism in Klebsiella pneumoniae}, keyword = {Glycerol dehydratase, Diol dehydratase, Microcompartment, 1, 3-propanediol, Klebsiella pneumoniae}, chapternumber = {110021} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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