Naslov
αSMA-Expressing Perivascular Cells Represent Dental Pulp Progenitors In Vivo

Autori
Vidovic Zdrilić, Ivana ; Banerjee, A. ; Fatahi, R. ; Matthews, B.G. ; Dyment, N.A. ; Kalajzic, I. ; Mina, M. Vidović, Ivana ; Banerjee, A. ; Fatahi, R. ; Matthews, B.G. ; Dyment, N.A. ; Kalajzic, I. ; Mina, M.

Izvornik
Journal of dental research (0022-0345) 96 (2016), 3; 323-390

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
odontoblasts, pulp biology, dentinogenesis, reparative dentin, stem cells, dentin sialophosphoprotein

Sažetak
The goal of this study was to examine the contribution of perivascular cells to odontoblasts during the development, growth, and repair of dentin using mouse molars as a model. We used an inducible, Cre-loxP in vivo fate-mapping approach to examine the contributions of the descendants of cells expressing the αSMA-CreERT2 transgene to the odontoblast lineage. In vivo lineage-tracing experiments in molars showed the contribution of αSMA-tdTomato cells to a small number of newly formed odontoblasts during primary dentinogenesis. Using an experimental pulp exposure model in molars to induce reparative dentinogenesis, we demonstrate the contribution of αSMA-tdTomato cells to cells secreting reparative dentin. Our results demonstrate that αSMA-tdTomato cells differentiated into Col2.3-GFP cells composed of both Dspp odontoblasts and Bsp osteoblasts. Our findings identify a population of mesenchymal progenitor cells capable of giving rise to a second generation of odontoblasts during reparative dentinogenesis. This population also makes a small contribution to odontoblasts during primary dentinogenesis

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

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