Naslov
Konvergencija agregiranih proteina prisutnih kod mentalnih bolesti i neurodegenerativnih poremećaja
(Convergence of aggregating proteins involved in mental illness and neurodegenerative disorders)

Autori
Gvoić, Ines

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad

Fakultet
Odjel za biotehnologiju

Mjesto
Rijeka

Datum
16.09

Godina
2019

Stranica
44

Mentor
Bradshaw, Nicholas James

Ključne riječi
mentalne bolesti, neurodegenerativne bolesti ; agregacija proteina ; DISC1 ; FUS ; TRIOBP-1
(mental illness ; neurodegenerative disease ; protein aggregation ; DISC1 ; FUS ; TRIOBP-1)

Sažetak
Schizophrenia and recurrent affective disorders like depression and bipolar disorder are neuropsychiatric conditions with poorly understood background and are some of the leading causes of disability worldwide. Existing diagnostic methods for these disorders rely entirely on a subjective psychiatric evaluation. Recent searches for biological markers of the diseases have identified protein aggregation and related changes in the protein homeostasis as potential factors in development of mental illness. Disturbed in Schizophrenia 1 (DISC1) is one of the proteins found in aggregates in the brain tissue of patients. DISC1 behaves as a scaffold protein that interacts with a variety of other proteins. DISC1 is known to co-aggregate with two proteins associated with neurodegenerative disorders, huntingtin and TDP-43, as well as other proteins implicated in mental illness. We have investigated the aggregation propensity and novel interactors of DISC1 in HEK293T cell culture using co- expression with another protein implicated in neurodegenerative disorders, Fused in Sarcoma protein (FUS). Microscopic examination of cells overexpressing full length DISC1 and FUS mutant types showed that DISC1 can be recruited to FUS protein aggregates. In similar conditions no aggregation was observed between FUS and Trio and F-actin binding protein 1 (TRIOBP-1), a schizophrenia related protein. We conclude that co-aggregation between DISC1 and FUS protein represents a potential direction in future research as one of the molecular processes involved in development of neuropsychiatric disorders.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA