Naslov
Testing the role of L-type Cryptochrome in circadian and circalunar light dependent behavior, in the marine bristle worm Platynereis dumerilii

Autori
Barbara Rodin

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Odjel za biotehnologiju i Max Perutz Lab Beč

Mjesto
Rijeka

Datum
28.09

Godina
2018

Stranica
70

Mentor
Rozi Andretić Waldowski

Neposredni voditelj
Kristin Tessmar-Raible

Ključne riječi
circadian clock, circalunar clock, L-cryptochrome, Platynereis dumerili, light entrainment

Sažetak
In order to maximize their chances of survival and procreation animals have to be able to predict and adapt to a range of periodic changes in their environment. To be able to anticipate environmental rhythms, like daynight illumination, they developed an endogenous timekeeping oscillators (circadian clocks), with approximately the same period length and phase as the external cycle they are subjected to. Endogenous clocks use external cues to entrain to the environment and produce behavioral and physiological outputs to help animals adjust accordingly. Light is the most prominent entrainment cue (zeitgeber) and light- responsive cryptochromes have been proven to facilitate entrainment in species like Arabidopsis thaliana, Soganus luridus and Drosophila melanogaster. Since marine habitats harbor a multitude of natural rhythms, marine organisms often have more than one type of clock. Marine annelid Platynereis dumerilii possess two clocks entrained by light: circadian clock, controlling their daily locomotor activity, and circalunar clock, controlling the timing of their sexual maturation. Considering P. dumerilii possess a light responsive Drosophila-type cryptochrome (L- cry), I hypothesized Lcry acts as a putative blue- light receptor facilitating clock function, so lossof-function animals (L-cry-/-) should display disruptions in circalunar and circadian behavioral and physiological outputs. I tested the hypothesis by performing a maturation pattern analysis to see how L-cry influences the circalunar rhythm, and multiple circadian experiments to test its circadian role. To confirm unpublished laboratory observations of L-cry-/- animals developing slower than their L-cry+/+ and Lcry+/- siblings I also performed a regeneration experiment. The experiments showed L-cry-/- exhibit a major maturation delay likely due to overall developmental delay especially in segment addition, regeneration and gametogenesis. L-cry likely does not play a dominant role in maturation itself, but mechanisms involved could be under circalunar or circadian influence. L-cry-/- also exhibited changes in timing of circalunar maturation as well as increase in arrhythmic circadian behavior and susceptibility to masking effects of light, which confirms my hypothesis that L-cry has a crucial role in circadian and circalunar clocks of P. dumerilii.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

Napomena
Https://repository.biotech.uniri.hr/en/islandora/obj
ect/biotechri:234

POVEZANOST RADA