Naslov
Use of bacterial Rho helicase to gain new insights into the targeting mechanism of nuclear RNAs by the exosome-associated exoribonuclease Rrp6 and its cofactors in yeast

Autori
Stuparevic Igor, Moreau Kevin, Rahmouni A. Rachid

Izvornik
Periodicum biologorum (0031-5362) (2020), 3-4; 147-153

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
bacterial Rho factor, yeast, mRNP biogenesis, mRNP quality control, RNA-seq, ChIP-seq

Sažetak
In eukaryotic cells, aberrant mRNPs with processing and packaging defects are targeted co- transcriptionally by a surveillance system that triggers their nuclear retention and ultimately the degradation of their mRNA component by the 3’-5’ activity of the exosome-associated exoribonuclease Rrp6 along with its associated factors Rrp47 and Mpp6. This mRNP quality control process is stimulated by the NNS complex (Nrd1-Nab3-Sen1), which otherwise mediates termination, processing and decay of ncRNAs and the process involves additionally the exosome co- activator TRAMP complex (Trf4-Air2-Mtr4). Here, we describe a genome-wide approach to visualize the dynamic recruitment of these quality control components over the yeast chromosomes upon global perturbation of mRNP biogenesis by the RNAdependent helicase/translocase activity of the bacterial Rho factor. The method provides valuable information on how the surveillance system coordinates with the transcription machinery in order to detect faulty events during perturbation of mRNP biogenesis. In addition, our overview shows that the assembly of the quality control components for the affected mRNA genes takes place at the expense of their commitment to be recruited to ncRNAs genomic features, which in turn leads to termination and processing defects of ncRNAs.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Biotehnologija

POVEZANOST RADA