Naslov
Kinetochore movement during chromosome congression depends on the formation of interpolar bundles

Autori
Štimac, Valentina ; Koprivec, Isabella ; Matković, Jurica ; Simunić, Juraj ; Tolić, Iva M.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Cell Bio Virtual 2020 - An Online ASCB|EMBO Meeting

Mjesto i datum
Philadelphia (PA), Sjedinjene Američke Države, 02.12.2020. - 16.12.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
Prometaphase ; Congression ; Bundles

Sažetak
During mitosis, the cell forms a spindle that equally segregates chromosomes into two daughter cells. Precise spatial organization of microtubule bundles and kinetochores is necessary for spindle function, yet it is unknown how unevenly distributed microtubules present during prometaphase organize into precise kinetochore and bridging fibers, important for positioning the kinetochores into the metaphase plate. By using live cell imaging of RPE1 cells stably expressing EYFP‐tubulin, we showed that in early prometaphase microtubules are organized in a few unevenly distributed and wide bundles. In contrast, late prometaphase and metaphase spindles are more ordered, consisting of numerous bundles that are similar in width and evenly spaced. Live cell imaging of HeLa cells stably expressing GFP‐tagged microtubule crosslinker PRC1 also revealed that in prometaphase, the spindle has a small number of unequally sized and spatially distributed PRC1‐ labeled overlap bundles, whose number increases either by forming de novo or exploiting the previously formed bundles. Depletion of PRC1 leads to different kinetochore movement during chromosome congression, which delays the formation of a stable metaphase plate. Thus, these results indicate a link between interpolar bundle formation and chromosome movements during congression in human cells.

Izvorni jezik
Engleski

POVEZANOST RADA