Naslov
The surface antigen CD45R identifies a population of estrogen-regulated murine marrow cells that contain osteoclast precursors.

Autori
Katavić, Vedran ; Grčević, Danka ; Lee, Sun Kyeong ; Kalinowski, Judoth ; Jastrzebski, Sandra ; Dougall, William ; Anderson, Dirk ; Puddington, Lynn ; Aguila, Leonardo H. ; Lorenzo, Joseph A.

Izvornik
Bone (8756-3282) 32 (2003), 6; 581-590

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
B-lymphocytes; Bone marrow; Bone resorption; Flow cytometry; Ovariectomy; Type 1 IL-1 receptor knockout mice

Sažetak
We examined the osteoclastogenic potential of murine bone marrow cells that were fractionated according to their expression of the surface antigen CD45R. Osteoclast-like cells (OCL) with many authentic osteoclast characteristics readily formed in purified CD45R+ murine bone marrow cell cultures after treatment with receptor activator of nuclear factor B ligand (RANKL) and M-CSF. Ovariectomy (Ovx) caused a 1.5- to 2-fold increase in OCL number in unfractionated and CD45R+ murine bone marrow cell cultures without affecting OCL formation in CD45R- marrow cells. Limiting dilution assays confirmed that Ovx caused an increase in osteoclast precursor cell number in CD45R+ but not CD45R- cells. Mice deficient in the type 1 IL-1 receptor (IL-1R1 KO) do not lose bone mass after Ovx. We found that unfractionated, CD45R+, and CD45R- bone marrow cells from IL-1R1 KO mice showed no increase in OCL formation in vitro after Ovx. In both the wild-type (WT) and the IL-1R1 KO mice Ovx was associated with a 2-fold increase in pre-B-lymphocytes. About 1.3 - 3.5% of murine marrow cells expressed surface RANK (the receptor for RANKL) while about 11.9 - 15% of murine bone marrow cells expressed c-Fms (the receptor for M-CSF). There was little effect of Ovx on cells expressing either RANK or c-Fms. These results demonstrate that CD45R expression identifies a subset of murine bone marrow cells whose ability to form OCL in vivo is regulated by estrogen in WT but not IL-1R1 KO cells. The effects of estrogen on bone mass may be related to these responses.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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