Naslov
Characterization, classification and localization of repetitive DNA sequences constituting the genome of the Pacific oyster (Crassostrea gigas)

Autori
Tunjić Cvitanić, Monika ; Vojvoda Zeljko, Tanja ; Pasantes Ludena, Juan Jose ; García-Souto, Daniel ; Plohl, Miroslav ; Šatović, Eva

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
45th FEBS Congress, Molecules of Life: Towards New Horizons, Ljubljana, Slovenia, July 3–8, 2021, Posters / - , 2021, 107-108

Skup
45th FEBS Congress, Molecules of Life: Towards New Horizons, Ljubljana, Slovenia, July 3–8, 2021

Mjesto i datum
Ljubljana, Slovenija, 03.07.2021. - 08.07.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bivalves, Crassostrea gigas, repetitive DNA, satellite DNA, centromeres, heterochromatin

Sažetak
Bivalve molluscs are a large group of economically and ecologically important invertebrates, which made them particularly interesting as model organisms for genomic research. So far, 31 bivalve genomes have been sequenced, the first one corresponding to the Pacific oyster (Crassostrea gigas). Segments of the genome enriched in repetitive sequences still present a challenge in the genome assemblies, leaving the composition of DNA sequences underlying the heterochromatic regions and the centromeres still unexplored in many organisms. The typical landmark of centromere chromatin is a variant of the H3 histone, CenH3, while DNA sequences building constitutive heterochromatin are associated with H3K9me3- histone modification. In order to investigate the DNA composition of genomic regions enriched in repetitive sequences, we employed chromatin immunoprecipitation followed by high-throughput next-generation sequencing of the whole genome and CenH3- and H3K9me3-associated sequences. The obtained sequences were analysed using RepeatExplorer pipeline. Fifty-two satellite DNAs constitute the satellitome. CenH3-associated sequences resulted in diverse repetitive DNA content and were assigned to six different groups of repetitive sequences. Several sequences enriched in CenH3-ChIP were localized on metaphase chromosomes using fluorescence in situ hybridization, each encompassing centromeres of only a few chromosomes of C. gigas. The same sequences were also colocalized with CenH3 on male gonadal cells in different stages of spermatogenesis, revealing stage-specific distribution of the CenH3. The heterochromatin of C. gigas exhibited low abundance and localization limited on two chromosomal pairs, with H3K9me3- associated sequences being dominantly composed of DNA transposons. To conclude, we identified, characterized and localized the most abundant repetitive sequences present in the genome of the Pacific oyster.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA