Naslov
Green extraction of flavonoids and phenolic acids from elderberry (Sambucus nigra L.) and rosemary (Rosmarinus officinalis L.) using deep eutectic solvents

Autori
Vladimir-Knežević, Sanda ; Perković, Marijana ; Zagajski Kučan, Kristina ; Mervić, Mateja ; Rogošić, Marko

Izvornik
Chemical papers (2585-7290) 76 (2022), 1; 341-349

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
deep eutectic solvent ; elderberry ; rosemary ; flavonoid ; phenolic acids

Sažetak
Recent research on the extraction of valuable natural compounds, especially plants antioxidants, was focused on the use of green solvents, including deep eutectic solvents (DES). For the first time, selected DES solvents were applied for the extraction of phenolic acids and favonoids from elderberry (Sambucus nigra L.) and rosemary (Rosmarinus ofcinalis L.). The presence of those polyphenolic compounds in elderberry fowers and rosemary leaves was detected by thin-layer chromatography, and their contents were determined spectrophotometrically. Extraction efciencies of water, 70% ethanol and DESs based on choline chloride (ChCl) and ethylene glycol (EG) in a molar ratio of 1:3 and choline chloride and glycerol (Gly) in the same molar ratio were compared. DESs were applied in pure form and with the addition of 30% and 50% of water (V/V). Obtained results showed that 50% ChCl:Gly and 50% ChCl:EG have similar efciency for phenolic acid extraction as commonly used 70% ethanol. The EG- based DES showed slightly higher performance for the extraction of rosemary phenolic acids, while the one based on Gly showed better results for elderberry. With respect to flavonoid extraction from elderberry fowers, 70% ChCl:EG was comparable to 70% ethanol. Our study demonstrated that tested novel and environmentally friendly DESs could replace ethanol for the extraction of plant polyphenols.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kemijsko inženjerstvo, Javno zdravstvo i zdravstvena zaštita, Farmacija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA