Naslov
Transcription factor CUX2 Is expressed in the interneurons of transient cellular compartments of the developing human neocortex

Autori
Miškić, Terezija ; Krsnik, Željka ; Kostović, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
8th Croatian neuroscience congress : book of abstracts / - Zagreb, 2021, 65-65

Skup
8th Croatian neuroscience congress

Mjesto i datum
Online, 24.09.2021. - 25.09.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
interneurons ; subplate ; neocortex

Sažetak
CUX2 is a transcription factor expressed in the neurons of differentiated upper cortical layers. CUX2 expression pattern throughout human fetal cortical development is mostly associated with pyramidal neurons of excitatory origin. CUX2 role in rodents is associated with synaptic development and molecular specification, while its role in human neocortical development is mostly unknown. Experiments on rodents already found that Cux2 is expressed in cortical interneurons that invade the pallium via tangential migration routes. Herein, we focused on the molecular characterization of CUX2 neuronal populations within the transient cellular compartments and possibly exploring its role during neurodevelopment. CUX2 protein co- expression with different neurotransmitter markers (NPY, GAD65/67, SST, nNOS) and calcium-binding protein calbindin is identified using immunofluorescence on formalin fixed paraffinembedded sections of postmortem human brains from 8 to 25 post-conceptional weeks (PCW) of the Zagreb Neuroembryological Collection. In the earliest fetal stage, a co-expression of CUX2 and calbindin is present, while CUX2 expression in the early stages is somewhat weak and there is no visible overlap with GAD65/67 expression. At 13 PCW, no co-localization of CUX2 nuclei and SST or GAD65/67 markers was identified neither in MZ nor in pSP. During the midgestation, NPY co-localized with CUX2 nuclei both in MZ and SP in the neocortex at 21 PCW. Furthermore, we found a co- localization between SST and CUX2 nuclei in the SP, but not in the MZ. nNOS neurotransmitter is expressed and co- localized with CUX2 in some SP cells, even though it is not expressed in the MZ. GAD65/67 expression is visible both in the MZ and SP cells, yet without the overlap with CUX2 positive nuclei. We showed CUX2 expression in the population of interneurons within the transient fetal compartments throughout the cortical development. Besides, we have already found CUX2 expressed in the postmigratory, the earliest differentiated neuronal population of the two transient cortical compartments: SP and MZ. CUX2 expression in postmigratory SP and MZ neurons, as well as the SP somatostatin neuronal population of the human fetal cortex, suggests a role for CUX2 in the formation of the first neuronal circuits.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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