Naslov
EBay and supplements don't mix: determination of andrographolides, boswellic acids, curcuminoids and piperine in products available locally and online

Autori
Brusač, Edvin ; Jeličić, Mario-Livio ; Amidžić Klarić, Daniela ; Nigović, Biljana ; Mornar, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
27th Croatian Meeting of Chemists and Chemical Engineers Book of Abstracts / Marković, Dean ; Meštrović, Ernest ; Namjesnik, Danijel ; Tomašić, Vesna - Zagreb : Hrvatsko kemijsko društvo, 2021, 35-35

Skup
27. hrvatski skup kemičara i kemijskih inženjera (27HSKIKI)

Mjesto i datum
Veli Lošinj, Hrvatska, 05.10.2021. - 08.10.2021

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
dietary supplements ; curcuminoids ; andrographolides ; boswellic acids ; piperine

Sažetak
Active substance content is one of the essential parameters of any preparation, as it often governs both efficacy and safety of the overall product. It is well known herbal products are not subjected to such rigorous control as conventional pharmaceuticals, so their quality can drastically differ. Therefore, the goal of this work was to assess the active substance content of turmeric, green chiretta and Indian frankincense-based products available locally and online. As these products are often comprised of two or more stated herbal constituents, a universal HPLCDAD method for determination of three curcuminoids, piperine (curcuminoid bioavailability enhancer), three andrographolides and six boswellic acids was developed and validated for the first time. Sonication extraction step was optimized using three-factorial Box-Behnken design, while the optimal parameters were extraction solvent of 81.5% (V/V) ethanol/water, sonication time of 30 min and extraction temperature of 60 °C (model desirability 0.927). The method was validated according to ICH guidelines, with selectivity (evaluated using standard addition method) and robustness of the method (evaluated using Plackett-Burman design) being paid special attention to. In total, 54 samples (19 raw material and 35 herbal preparation) were analyzed. Drastic differences in declared content of herbal preparations (1.4 to 176.8%) were observed. Regarding raw material, two online-marketed samples showed suspiciously high amounts of curcuminoids, pointing to possible adulteration. Overall, most online-marketed products showed either very little active substances (39% of Internet-bought preparation samples showed less than 20% declared content, implying no efficacy) or substantially higher amounts which could potentially cause adverse effects (one sample containing andrographolides) or lead to an interaction with concomitantly used drugs (one sample containing piperine). In conclusion, consumers should purchase dietary supplements and similar products from certified manufacturers in local pharmacies and refrain from buying them online.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA