Naslov
Evaluation of thermodynamic parameters between antidepressant imipramine and human alpha1-acid glycoprotein by isothermal titration calorimetry
(Evaluation of thermodynamic parameters between antidepressant imipramine and human alpha1-acid glycoprotein by isothermal titration calorimetry)

Autori
Kerep, Robert ; Šeba, Tino ; Gabričević, Mario

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
27. HSKIKI 2021. - knjiga sažetaka / - , 2021, 1-1

Skup
27. hrvatski skup kemičara i kemijskih inženjera (27HSKIKI)

Mjesto i datum
Veli Lošinj, Hrvatska, 05.10.2021. - 08.10.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
alpha-acid glycoprotein ; plasma protein binding ; ITC

Sažetak
α1-acid glycoprotein (AGP, also known as orosomucoid) is an important plasma protein involved in the binding and transport of many drugs, especially basic compounds [1]. This research uses a novel sensitive and modern isothermal titration calorimetry (ITC) technique for characterization of microscopic thermodynamic parameters that trigger the binding of drugs to AGP [2]. This assay is a convenient and widely used approach to directly measure the amount of heat released or absorbed during association processes of biomolecules, such as protein-drug, in solution and to quantitatively estimate the interaction affinity. The binding of basic drug imipramine displayed an exothermically driven binding interaction with AGP. The exothermic binding energy of compounds were guided by a combination of favorable (negative) enthalpy (ΔrH = –11.2 kcal/mol) and favorable (positive) entropy (ΔrS = 4.62 kcal/mol K) contribution to the Gibbs free energy (ΔrG = –6.57 kcal/mol) with dissociation constant (KD = 23.7 "μ" M). By accessing the enthalpy change, we get a step closer in estimating the driving forces that characterize the interaction of a protein with a drug, information much needed in the drug discovery process.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA