Naslov
Grass pea natural variation reveals oligogenic resistance to Fusarium oxysporum f. sp. pisi

Autori
Sampaio, Ana Margarida ; Alves, Mara Lisa ; Pereira, Priscila ; Valiollahi, Ehsan ; Santos, Carmen ; Šatović, Zlatko ; Rubiales, Diego ; de Sousa Araújo, Susana ; van Eeuwijk, Fred ; Vaz Patto, Maria Carlota

Izvornik
Plant Genome (1940-3372) 2021 (2021); E20154, 19

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Lathyrus sativus L., SNP, GWAS, fusarium wilt, resistance

Sažetak
Grass pea (Lathyrus sativusL.) is an annual legume species, phylogenetically closeto pea (Pisum sativumL.), that may be infected byFusarium oxysporumf. sp.pisi(Fop), the causal agent of fusarium wilt in peas with vast worldwide yield losses.A range of responses varying from high resistance to susceptibility to this pathogenhas been reported in grass pea germplasm. Nevertheless, the genetic basis of thatdiversity of responses is still unknown, hampering its breeding exploitation. To iden-tify genomic regions controlling grass pea resistance to fusarium wilt, a genome-wide association study approach was applied on a grass pea worldwide collection ofaccessions inoculated withFoprace 2. Disease responses were scored in this col-lection that was also subjected to high-throughput based single nucleotide polymor-phisms (SNP) screening through genotyping-by-sequencing. A total of 5, 651 high-quality SNPs were considered for association mapping analysis, performed usingmixed linear models accounting for population structure. Because of the absence of a fully assembled grass pea reference genome, SNP markers’ genomic positionswere retrieved from the pea’s reference genome v1a. In total, 17 genomic regionswere associated with three fusarium wilt response traits in grass pea, anticipating anoligogenic control. Seven of these regions were located on pea chromosomes 1, 6, and 7. The candidate genes underlying these regions were putatively involved in sec-ondary and amino acid metabolism, RNA (regulation of transcription), transport, anddevelopment. This study revealed important fusarium wilt resistance favorable grasspea SNP alleles, allowing the development of molecular tools for precision diseaseresistance breeding.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija), Biotehnologija

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