Naslov
BPM1 protein is involved in plant development and stress responses by regulating de novo DNA methylation

Autori
Bauer, Nataša ; Markulin, Lucija ; Jagić, Mateja ; Vuk, Tamara ; Vitko, Sandra ; Škiljaica, Andreja ; Tokić, Mirta ; Vičić-Bočkor, Vedrana ; Vidaković-Cifrek, Željka ; Leljak-Levanić, Dunja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
45th FEBS Congress: Molecules of Life: Towards New Horizons (FEBS 2021)

Mjesto i datum
Ljubljana, Slovenija, 03.07.2021. - 08.07.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Arabidopsis thaliana, metilacija DNA, BPM1, proteasomalna degradacija
(Arabidopsis thaliana, DNA methylation, BPM1, proteasomal degradation)

Sažetak
A member of a small family of Arabidopsis thaliana MATH-BTB proteins, BPM1 functions as a substrate-specific adaptor of a cullin3-based E3 ubiquitin ligase complex. Its N-terminal MATH domain binds target proteins and designates them for ubiquitination and subsequent proteasomal degradation. Recent studies reported that BPM1 mediates proteasomal degradation of transcription factors (ERF/AP2, R2R3 MYB and Homeobox) and phosphatases (PP2Cs). By using immunoprecipitation, TAP-tag purification, fluorescence-lifetime imaging microscopy, yeast-2-hybrid, pull down and microscale thermophoresis we confirmed BPM1 interaction with DMS3 and RDM1, key regulators of de novo DNA methylation in A. thaliana. Chromatin immunoprecipitation was used to determine regions of overlap between DMS3 and BPM1 chromatin binding sites. The DNA methylation status of identified regions was analysed by pyrosequencing after bisulphite conversion in A. thaliana plants overexpressing BPM1, in plants with downregulated BPMs as well as in DNA methylation mutants. Methylation patterns were assessed during plant development and after exposure to different abiotic stresses. Our results indicate that BPM1 does not participate in degradation of DMS3 and RDM1. Rather, it seems BPM1 recruits DNA methylation machinery to specific chromatin positions for de novo DNA methylation establishment.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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