Naslov
Changes in activities and isoform patterns of antioxidant enzymes in tobacco plants upon exposure to silver nanoparticles and silver nitrate

Autori
Košpić, Karla ; Biba, Renata ; Cvjetko, Petra ; Peharec Štefanić Petra ; Balen Biljana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
4th INPPO Virtual Conference International Plant Proteomics Organization (INPPO)

Mjesto i datum
Online; konferencija, 09.03.2021. - 11.03.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antioxidant enzymes, phytotoxicity, silver nanoparticles

Sažetak
With the rapidly growing utilization of nanomaterials in industry and various consumer products comes a great environmental health risk. Due to their well-known antimicrobial properties, silver nanoparticles (AgNPs) are the most exploited nanomaterial, particularly in agriculture and food production. However, being released to water or soil, AgNPs are likely to interact with plants and thereby enter the food chain. In this work, in vitro grown tobacco (Nicotiana tabacum) plants were exposed to either AgNPs stabilized with cetyltrimethylammonium bromide (CTAB) or polyvinylpyrrolidone (PVP) coating or to ionic silver (AgNO3) of the same concentrations (25, 50 and 100 μM) in order to determine changes in the antioxidant enzymes system. Spectrophotometric enzyme assays were used to determine the activities of antioxidant enzymes catalase (CAT), ascorbate (APX) and pyrogallol peroxidase (PPX) and superoxide dismutase (SOD) in leaf extracts of exposed and control plants, followed by polyacrylamide gel electrophoresis in native conditions, applied to determine different isoenzymes and changes in expression patterns in regard to different treatments. Immunoblotting was used to identify each individual antioxidant enzyme and analyse their expression levels. The results showed that the most prominent changes in activities of SOD and PPX were observed after exposure to AgNO3 treatments, while 100 µM AgNP- CTAB showed the highest increase in both CAT and SOD activities. The form of applied silver seemed to affect the expression of different isoenzymes, with the most notable differences observed in APX and PPX isoform patterns when comparing exposure to ionic silver and AgNP treatments. Exposure to AgNP-CTAB resulted with the highest increase in all antioxidant enzyme levels compared to other treatments of the same concentration, according to Western blot results. Obtained results suggest that oxidative stress is associated with AgNPs toxicity in plants, and cannot be ascribed only to dissolution of silver ions.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA