Pregled bibliografske jedinice broj: 1150035
Royal Jelly and Human Interferon-Alpha (HuIFN-αN3) Affect Proliferation, Glutathione Level, and Lipid Peroxidation in Human Colorectal Adenocarcinoma Cells In Vitro
Royal Jelly and Human Interferon-Alpha (HuIFN-αN3) Affect Proliferation, Glutathione Level, and Lipid Peroxidation in Human Colorectal Adenocarcinoma Cells In Vitro // Lipid Peroxidation Research / Mansour, Mahmoud Ahmed (ur.).
London : Delhi: IntechOpen, 2020. str. 65-76 doi:10.5772/intechopen.85777
CROSBI ID: 1150035 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Royal Jelly and Human Interferon-Alpha (HuIFN-αN3)
Affect Proliferation, Glutathione Level, and Lipid
Peroxidation in Human Colorectal Adenocarcinoma
Cells In Vitro
Autori
Filipič, Bratko ; Gradišnik, Lidija ; Rihar, Klemen ; Pereyra, Adriana ; Đermić, Damir ; Mazija, Hrvoje
Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni
Knjiga
Lipid Peroxidation Research
Urednik/ci
Mansour, Mahmoud Ahmed
Izdavač
IntechOpen
Grad
London : Delhi
Godina
2020
Raspon stranica
65-76
ISBN
978-1-83968-547-7
Ključne riječi
antiproliferative activity, antitumor activity, CaCo-2 cells, 10-hydorxy-2-decenoic acid, malondialdehyde, HuIFN-αN3, RJ-F (M)
Sažetak
The purpose was to investigate the influence of RJ-F(M), 10-hydroxy-2- decenoic acid and HuIFN-αN3 on the proliferation of CaCo-2 cells and ascertain their effects on intracellular glutathione level and lipid peroxidation. The antiproliferative (AP) activity of RJ-F (M) (0.1 g/10 mL PBS), HuIFN-αN3 (1000 IU mL−1), 10-HDA (100.0 μmol L−1) and their combinations, in the ratios 1:1, 1:2, and 2:1 on CaCo-2 cells were measured. Single RJ-F (M) had a low AP activity: 2.0 (0.5 mg mL−1). HuIFN-αN3 had an AP activity of 2.5 (208.33 IU mL−1), while 10-HDA had an AP activity of 1.5 (37.5 μmol mL−1). AP activity of 3.8 was obtained when RJ-F(M) and HuIFN-αN3 were in the ratio 2:1. In it, the level of GSH was 24.9 ± 2.4 nmol g−3 of proteins (vs. 70.2 ± 3.2 nmol g−3 in the control), and level of MDA was 72.3 ± 3.1 nmol g−3 (vs. 23.6 ± 9.1 nmol g−3 in the control). 10-HDA, together with HuIFN-αN3, is responsible for the inhibition of CaCo-2 cell proliferation in vitro. RJ-F (M) and HuIFN-αN3 applied at 2:1 decreased level of GSH and increased lipid peroxidation via MDA in CaCo-2 cells. Future studies are needed whether these GSH- and MDA-related activities of RJ-F (M), HuIFN-αN3, 10-HDA, and their combinations may decrease the tumorigenicity index and tumorigenic potential of tumor cells in vitro.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
POVEZANOST RADA
Ustanove:
Institut "Ruđer Bošković", Zagreb
