Naslov
Human Papillomaviruses in Cervical Cancer: Biomarkers in Clinical Practice and Research

Autori
Milutin Gašperov, Nina ; Fureš, Rajko ; Erceg Ivkošić, Ivana ; Malinac Malojčić, Sanja ; Pešut, Ena ; Šimić, Ivana ; Gredičak, Martin ; Jakopović, Marija Magdalena ; Feratović, Fabijan ; Sabol, Ivan ; Grce, Magdalena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
III. Kongres Hrvatskog društva za pelviperineologiju s međunarodnim sudjelovanjem i II. Poslijediplomski tečaj zdjelične medicine : knjiga sažetaka / Hrvatsko društvo za pelviperineologiju - , 2021, 26-26

Skup
3. kongres hrvatskog društva za pelviperineologiju i tečaj Zdjelična medicina

Mjesto i datum
Poreč, Hrvatska, 01.10.2021. - 03.10.2021

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
HPV ; cervical cancer ; biomarkers

Sažetak
High-risk (HR) subtypes of the human papilloma virus (HPV) are the cause of the cervical cancer (CC) in most cases. Alongside implementation of HPV vaccines there is still need for effective and accessible cervical screening, better diagnostic tools and stronger implementation processes to reach even more women and save more lives. Besides primary Pap smear as a diagnostic tool in CC screening, HPV DNA testing has been considered and assessed as a primary screening method in larger population-based studies. The most common HPV testing is the detection of HR HPV DNA, either by DNA amplification or by hybridization. Testing for HPV viral oncogenes E6 and E7 mRNA is clinically even more specific and informative evidence of active viral infection. In addition, overexpressed p16 being a surrogate biomarker for persistent infection with HR HPV types serves for triage of undetermined cytology findings. Ki-67 expression indicating cell proliferative capacity is in line with increasing grades of cervical dysplasia, and represents, together with p16, a valuable biomarker in immunohistochemistry. Furthermore, epigenetic biomarkers have become of considerable interest, especially DNA methylation and micro RNA (miRNA) profiling. DNA methylation profiles can be used as a biomarker for distinguishing between normal mucosa, cervical lesions, and CC. The precancerous lesions, particularly CIN2+ lesions, can be detected by changes in DNA methylation profiles. Cellular miRNAs have been shown to be deregulated in CC, while no HPV encoded miRNAs were found. The advantages of miRNA profiling are both specificity and sensitivity, and the possibility to investigate different easily accessible body fluids and tissues. Both epigenetic biomarkers mentioned above represent future promising CC diagnostic and prognostic biomarkers.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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