Pregled bibliografske jedinice broj: 1149406
Formulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches
Formulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches // European journal of pharmaceutical sciences, 166 (2021), 105906, 11 doi:10.1016/j.ejps.2021.105906 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1149406 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Formulation of olopatadine hydrochloride viscous
eye drops – physicochemical, biopharmaceutical and
efficacy assessment using in vitro and in vivo
approaches
Autori
Račić, Andjelka ; Čalija, Bojan ; Milić, Jela ; Jurišić Dukovski, Bisera ; Lovrić, Jasmina ; Dobričić, Vladimir ; Micov, Ana ; Vuković, Milja ; Stepanović-Petrović, Radica ; Krajišnik, Danina
Izvornik
European journal of pharmaceutical sciences (0928-0987) 166
(2021);
105906, 11
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
HCE-T cell-based models ; MTT cytotoxicity assay ; ocular itch test ; olopatadine hydrochloride ; PAMPA ; viscous eye drops
Sažetak
The aim of this work was the formulation and the comprehensive evaluation of the viscous eye drops using vehicles containing medium chain chitosan (0.5% w/v), hydroxypropyl guar gum (0.25% w/v) and their combination as carriers for olopatadine (0.1% w/v). Physicochemical properties (appearance, clarity, pH, osmolality, viscosity and drug content) of the tested formulations were within acceptable ranges for the ophthalmic preparations, while DSC and FT-IR techniques demonstrated the compatibility between olopatadine and polymers. The drug permeability was successfully estimated in vitro using both HCE-T cell-based models (Model I and Model II) and the parallel artificial membrane permeability assay (PAMPA), considering the impact of chitosan as a permeation enhancer. The MTT cytotoxicity assay demonstrates that the tested formulations (diluted 10-fold in HBSS pH 5.5) were non-toxic and well tolerated. An ocular itch test on mice was carried out with the formulation containing the combination of polymers comparable with a commercially available olopatadine eye drops without viscosity enhancers. The tested eye drops produced a slightly higher anti- pruritic/analgesic-like effect than the commercial preparation. It could be assumed that the use of this viscous ophthalmic vehicle due to its advanced mucoadhesive properties and good safety profile is a feasible strategy to improve the efficacy of olopatadine.
Izvorni jezik
Engleski
Znanstvena područja
Farmacija
POVEZANOST RADA
Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
