Naslov
DIETARY n-3 PUFAs INCREASE ANTI-INFLAMMATORY CYTOKINE TGF-β1 PRODUCTION BY PBMCs FOLLOWING PMA/IONOMYCIN STIMULATION

Autori
Kolobarić, Nikolina ; Drenjančević, Ines ; Matić, Anita ; Šušnjara, Petar ; Mihaljević, Zrinka ; Mihalj, Martina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
15th Annual Meeting of Croatian Physiological Society wih International Participation Program and Abstract Book / - , 2021, 47-47

Skup
15th Annual Meeting of Croatian Physiological Society with International Partcipation

Mjesto i datum
Zagreb, Hrvatska, 07.10.2021. - 08.10.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
inflammation, functional food, PBMCs, cytokines

Sažetak
Introduction: Fatty acid derived lipid mediators have an important role in maintaining cytokine environment through direct effects on cytokine production and T lymphocytes. In the present study, we aimed to determine the effects of n-3 PUFA supplementation (~1053 mg/per day) through consumption of enriched hen eggs, on pro- and anti-inflammatory markers in healthy individuals (N=40 ; 23.8 ± 2.57 years old). Materials and Methods: Participants consumed three regular (N=21) or enriched hen eggs (N=19) per day through three weeks. Mononuclear cells (PBMCs) were isolated from whole blood by density gradient centrifugation and cultured in RPMI-1640 media (10% FBS ; 1% antibiotic). Concentrations of anti-/pro-inflammatory cytokines (IL-10, IL-6, IL- 23, IL-17A, and TGF-β1) and MCP-1 chemokine in both control and n-3 PUFA group, before and after dietary protocols were determined in supernatants following PMA/ionomycin stimulation with Invitrogen ProcartaPlex antibody-based, magnetic bead reagent kits on the Luminex 200 instrument platform. Results: TGF-β1 production by PBMCs following n-3 PUFA dietary protocol was significantly increased (p=0.026), while IL-6 production was significantly decreased (p=0.05), compared to the respective baseline levels. Target cytokine and chemokine production was unaffected by the consumption of regular hen eggs. Conclusion: The results of the present study have demonstrated that dietary n-3 PUFAs can modify key markers of inflammation and lead towards inflammation resolving environment in the population of young, healthy individuals.

Izvorni jezik
Engleski

Napomena
The study was funded by European Structural and
Investment Funds to Science Centre of Excellence
for Personalised Health Care, the Josip Juraj
Strossmayer University of Osijek, Scientific Unit
for Research, Production and Medical Testing of
Functional Food, # KK.01.1.1.01.0010.

POVEZANOST RADA