Naslov
Estimation of urinary cotinine cut-off value to discern pregnant at term cigarette smokers from non-smokers

Autori
Brčić Karačonji, Irena ; Jurič, Andreja ; Brajenović, Nataša ; Stasenko, Sandra ; Mioč, Tatjana ; Miškulin, Iva ; Škrgatić, Lana ; Jurasović, Jasna ; Piasek, Martina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 6th Croatian Congress of Toxicology with International Participation CROTOX 2021 ; u: Arhiv za higijenu rada i toksikologiju 72 (2021) (S1) / Lyons, Daniel Mark - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2021, 50-50

Skup
6th Croatian congress of toxicology with international participation (CROTOX 2021)

Mjesto i datum
Rabac, Hrvatska, 03.10.2021. - 06.10.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
biomarker of cigarette smoking ; gas chromatography-mass spectrometry ; ROC curve ; solid phase microextraction ; spot urine.

Sažetak
Prenatal exposure to cigarette smoking has been extensively studied as one of the factors that may have adverse health outcomes later in life. Reliance on self-reported smoking behaviour may lead to understatements of nicotine exposure due to negative social connotations related to maternal smoking. To overcome the uncertainty of self-reporting, cotinine, the major metabolite of nicotine in urine, is widely used as a reliable biomarker of tobacco smoke exposure. Since half- life of cotinine is much shorter in pregnant (8.8 h) vs. non-pregnant women (16.6 h), cut-off level to classify non-smokers and active smokers must be established for pregnancy. The aim of this study was to determine a cut-off value to discern pregnant non-smokers from pregnant smokers. Spot urine samples from pregnant women self-reported as non-smokers (n = 117 ; never smoked or smoked >12 months before the last pregnancy) or smokers (n = 39, smoking any time during pregnancy or within 12 months before last pregnancy) were collected in the maternity ward before delivery. Cotinine was extracted from urine by headspace solid phase microextraction and quantified using gas chromatography-mass spectrometry. A receiver operating characteristic (ROC) curve analysis showed that the optimal cut-off value of urinary cotinine to discern pregnant non-smokers from smokers was 24.1 μg/L (42.6 μg/g creatinine). Misclassification rate for current smokers who reported no smoking was 5.1 %. The presented cut- off value for urinary cotinine ensures a more accurate categorisation of the smoking habit among pregnant women than by self-reporting. The study was funded by the Croatian Science Foundation (grant HRZZ-IP-2016-06-1998).

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kliničke medicinske znanosti

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