Naslov
Tar DNA-binding protein 43 regulation in mice spinal cord during the first week following repetitive mild traumatic brain injury

Autori
Rajič Bumber, Jelena ; Pilipović, Kristina ; Gržeta, Nika ; Janković, Tamara ; Dolenec, Petra ; Križ, Jasna ; Župan, Gordana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
8th Croatian Neuroscience Congress

Mjesto i datum
Zagreb, Hrvatska, 24.09.2021. - 25.09.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Brain Concussion ; Mice ; TDP-43 Proteinopathies

Sažetak
Introduction: Repetitive mild traumatic brain injury (mTBI) is a risk factor for the development of different neurodegenerative diseases, including amyotrophic lateral sclerosis which is characterized by TAR DNA-binding protein 43 (TDP- 43) proteinopathy in most cases. The aim of this research was to examine intracellular regulation of TDP-43 in the spinal cord during the first week after repetitive mTBI in mice. Materials and methods: Repetitive mTBI was done by the weight drop method (Kane et al. 2012) twice a day, for five consecutive days in C57BL/6 mice. The control group of mice was subjected to a sham procedure, had the same handling and anesthesia, but received no impact. The mice were sacrificed one, three or seven days after the final injury/sham procedure and their spinal cords were dissected and prepared for the Western blot analyses of proteins of interest. Results: In the spinal cords of injured mice, increased cytoplasmatic expression of the phosphorylated TDP-43 was detected on the first day after repetitive mTBI, while the expression of TDP-43 and its cleavage product TDP-35, as well as of the phosphorylated TDP-35 and TDP-25 were not impaired at any of time points investigated. Conclusions: Our preliminary results suggest early and transient dysregulation of TDP-43 in the cytoplasm of the spinal cord cells in mice following repetitive mTBI, which are probably the result of an acute response to stress caused by the brain trauma.

Izvorni jezik
Engleski

POVEZANOST RADA