Naslov
Quantitative Determination of Metals in Honey Samples by TLC with Video Densitometry

Autori
Horvat, Alka J.M. ; Poljak, Višnja ; Šoljić, Zvonimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
New Achievements in Chromatography / Smolec, Sonja ; Rogošić, Marko - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2000

Skup
6th international Symposium New Achievements in Chromatography

Mjesto i datum
NP Plitvička jezera, Hrvatska, 11.10.2000. - 13.10.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
metal ions; TLC; determination; honey

Sažetak
Trace elements concentraton in honey can be used as an environmental pollution indicator, characterising the level of soil, plant and air pollution in the area of several square kilometers. This work reports of qualitative and quantitative determination of metals in honey by thin layer chromatoggraphy. The method comprises sample digestion in a microwave oven with nitric acid and hydrogen poroxide, separation of the samples on the TLC RP-18 pre-coated layers (Merck) in isopentanol-methanol-acetonitrile-water- HCl developer and quantification by CCD video densitometry. The developed chromatogram was visualised by spraying with dithisone and PAN solutions producing coloured spots on the yellow background. CAMAG Video dokumentation system porformed image capturing and data evaluation. Each image was quantitatively evaluated after compensation of the yellow background using CAMAG Video Scan 1.01 software.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA