Naslov
Melittin stability in honey bee venom under different storage conditions measured with RP- HPLC-PDA method

Autori
Flanjak, Ivana ; Primorac, Ljiljana ; Cvijetić Stokanović, Milica ; Puškadija, Zlatko ; Bilić Rajs, Blanka ; Kovačić, Marin

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Zbornik radova 56. hrvatskog i 16. međunarodnog simpozija agronoma / Vlatka, Rozman ; Zvonko, Antunović - Osijek : Fakultet agrobiotehničkih znanosti Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2021, 467-471

Skup
56. hrvatski i 16. međunarodni simpozij agronoma

Mjesto i datum
Vodice, Hrvatska, 05.09.2021. - 10.09.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
honey bee venom ; melittin ; RP-HPLC-PDA method ; stability ; storage

Sažetak
The aim of this study was to determine performance characteristics of reversed-phase high performance liquid chromatographic method with photo-diode array detector (RP-HPLC-PDA) for melittin content determination, and to evaluate the melittin content in honey bee venom during 6 months of storage at room temperature and in the freezer. Based on the obtained results of the method performance characteristics(linearity, precision, trueness, LOD and LOQ), the used RP-HPLC-PDA method is fit for purpose. Linearity was confirmed with correlation coefficient of 0.999 while relative standard deviation (RSD) of sample preparation repeatability was 2.45 %. Limits of detection and quantification were 0.62 μg/mL and 1.88 μg/mL, respectively. Statistical analysis showed that the storage temperature had no effect on the melittin content during 6 months of storage. The results of this study can be of great interest to the beekeepers because it show that venom quality is maintained with reduced cost for storing and manipulation.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Prehrambena tehnologija

POVEZANOST RADA