Pregled bibliografske jedinice broj: 1144060
Metallothioneins and Megalin Expression Profiling in Premalignant and Malignant Oral Squamous Epithelial Lesions
Metallothioneins and Megalin Expression Profiling in Premalignant and Malignant Oral Squamous Epithelial Lesions // Cancers, 13 (2021), 18; 1282383, 18 doi:10.3390/cancers13184530 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1144060 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Metallothioneins and Megalin Expression
Profiling in Premalignant and Malignant Oral
Squamous Epithelial Lesions
Autori
Zulijani, Ana ; Dekanić, Andrea ; Ćabov, Tomislav ; Jakovac, Hrvoje
Izvornik
Cancers (2072-6694) 13
(2021), 18;
1282383, 18
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
metallothioneins ; megalin ; interaction ; oral squamous cell carcinoma ; oral leukoplakia ; lichen planus ; regulated intramembrane proteolysis
Sažetak
This study aimed to assess the relationship and possible interactions between metallothioneins (MTs) and megalin (LRP-2) in different grades of oral squamous cell carcinoma (OSCC) and premalignant lesions of the oral mucosa (oral leukoplakia and oral lichen planus). The study included archived samples of 114 patients and control subjects. Protein expression was examined by immunohistochemistry and immunofluorescence, and staining quantification was performed by ImageJ software. Protein interaction in cancer tissue was tested and visualized by proximity ligation assay. Mann- Whitney and Kruskal-Wallis tests were used to determine the significance of differences between each group, whereas Pearson correlation coefficient was performed to test correlation. Expression of both proteins differed significantly between each group showing the same pattern of gradual increasing from oral lichen planus to poorly differentiated OSCC. Moreover, MTs and megalin were found to co- express and interact in cancer tissue, and their expression positively correlated within the overall study group. Findings of prominent nuclear and chromosomal megalin expression suggest that it undergoes regulated intramembrane proteolysis upon MTs binding, indicating its ability to directly affect gene expression and cellular division in cancer tissue. The data obtained point to the onco- driving potential of MTs-megalin interaction.
Izvorni jezik
Engleski
Znanstvena područja
Dentalna medicina, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
POVEZANOST RADA
Ustanove:
Medicinski fakultet, Rijeka,
Klinički bolnički centar Rijeka,
Fakultet dentalne medicine, Rijeka
