Naslov
Current laboratory and clinical practices in reporting and interpreting anti-nuclear antibody indirect immunofuorescence (ANA IIF) patterns: results of an international survey

Autori
Van Hoovels, Lieve ; Broeders, Sylvia ; Chan, Edward K. L ; Andrade, Luis ; Cruvinel, Wilson de Melo ; Damoiseaux, Jan ; Viander, Markku ; Herold Manfred ; Coucke, Wim ; Heijnen, Ingmar ; Bogdanos, Dimitrios ; Calvo-Alén, Jaime ; Eriksson, Catharina ; Kozmar, Ana ; Kuhi, Lisa ; Bonroy, Carolien ; Lauwerys, Bernard ; Schouwers, Sofie ; Lutteri, Laurence ; Vercammen, Martine ; Mayer, Miroslav ; Patel, Dina ; Egner, William ; Puolakka, Kari ; Tešija Kuna, Andrea ; et al.

Izvornik
Autoimmunity Highlights (2038-0305) 11 (2020), 17; 1-12

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, stručni

Ključne riječi
Antinuclear antibodies ; ANA patterns ; Indirect immunofuorescence ; ICAP

Sažetak
Background: The International Consensus on Antinuclear Antibody (ANA) Patterns (ICAP) has recently proposed nomenclature in order to harmonize ANA indirect immunofuorescence (IIF) pattern reporting. ICAP distinguishes competent-level from expert-level patterns. A survey was organized to evaluate reporting, familiarity, and considered clinical value of ANA IIF patterns. Methods: Two surveys were distributed by European Autoimmunity Standardization Initiative (EASI) working groups, the International Consensus on ANA Patterns (ICAP) and UK NEQAS to laboratory professionals and clinicians. Results: 438 laboratory professionals and 248 clinicians from 67 countries responded. Except for dense fne speck‑ led (DFS), the nuclear competent patterns were reported by>85% of the laboratories. Except for rods and rings, the cytoplasmic competent patterns were reported by>72% of laboratories. Cytoplasmic IIF staining was considered ANA positive by 55% of clinicians and 62% of laboratory professionals, with geographical and expertise- related diferences. Quantifcation of fuorescence intensity was considered clinically relevant for nuclear patterns, but less so for cytoplas‑ mic and mitotic patterns. Combining IIF with specifc extractable nuclear antigens (ENA)/dsDNA antibody testing was considered most informative. Of the nuclear competent patterns, the centromere and homogeneous pattern obtained the highest scores for clini‑ cal relevance and the DFS pattern the lowest. Of the cytoplasmic patterns, the reticular/mitochondria-like pattern obtained the highest scores for clinical relevance and the polar/Golgi-like and rods and rings patterns the lowest. Conclusion: This survey confrms that the major nuclear and cytoplasmic ANA IIF patterns are considered clinically important. There is no unanimity on classifying DFS, rods and rings and polar/Golgi-like as a competent pattern and on reporting cytoplasmic patterns as ANA IIF positive.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Farmacija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA