Naslov
Embryotoxicity of dental restorative materials

Autori
Majstorović Matejić, Matea ; Babić, Sanja ; Par, Matej ; Tarle, Zrinka ; Čož-Rakovac, Rozalindra ; Marović, Danijela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of 6th International Congress of the School of Dental Medicine University of Zagreb. U: Acta stomatologica Croatica 54 (2) / Klarić Sever, Eva - , 2020, 221-221

Skup
6. međunarodni kongres Stomatološkog fakulteta Sveučilišta u Zagrebu = 6th International Congress of the School of Dental Medicine University of Zagreb

Mjesto i datum
Zagreb, Hrvatska, 28.02.2020. - 29.02.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
toxicity ; dental materials ; composite ; amalgam

Sažetak
Introduction: Final adjustment and polishing or removal of dental fillings produce particles of restorative materials of various sizes that can reach the wastewater. The purpose of this study is to investigate the effect of particles of restorative materials and their degradation products on the environment and non-target organisms using Danio rerio zebrafish embryos. Materials and methods: The study included dental amalgam (Amalcap plus, Ivoclar Vivadent), commercial composite (Tetric EvoCeram, Ivoclar Vivadent ; A2) and laboratory composite (BisGMA / TEGDMA, 70wt% filler). After polymerization of the composite materials (Bluephase G2, Ivoclar Vivadent ; 1200mW / cm2, 10s), or hardening of the amalgam (1h), micrometer particles were made and suspended in artificial water at various dilutions. Samples were continuously shaken during 48-h and 7-days incubation, followed by determination of embryotoxicity using Danio rerio embryos. Within 96 h of embryonic development on tested samples, lethal and sublethal (developmental abnormalities, cardiotoxicity, melanogenesis, etc.) endpoints were monitored. Results: All tested samples revealed high embryotoxicity. The commercial composite showed the lowest toxicity (1.5g of the composite caused 90% mortality, while in the other tested materials the same effect was observed already during the 250mg test). The laboratory composite showed higher toxicity in comparison to the commercial one, but its toxicity was reduced after 7 days of incubation compared to 48-h. Unlike the composite, the amalgam showed increased toxicity after 7 days of incubation (100%) versus 48-h (60%). High mortality on amalgam was present at all tested dilutions (0.25-4g). Conclusion: The highest percentage of composite-induced embryotoxicity was observed after 48 h of material incubation, while the highest percentage of amalgam-induced mortality was recorded after 7-days of material incubation. The obtained results emphasize the importance of such research and contribute to the establishment of biomarkers at different levels of biological organization in order to determine their safety for environment and non-target organisms.

Izvorni jezik
Hrvatski, engleski

Znanstvena područja
Dentalna medicina

POVEZANOST RADA