Naslov
Proteomic Analyses of Gingival Tissue in Patients with Aggressive Periodontitis

Autori
Badovinac, Ana ; Razdorov, Genadij ; Grgurević, Lovorka ; Plančak, Darije ; Vukičević, Slobodan ; Božić, Darko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
IADR Pan European Region Meeting. U: Journal of Dental Research. 2014 ; 93 Special Issue C:266 / - , 2014, 266-266

Skup
IADR Pan European Region Meeting

Mjesto i datum
Dubrovnik, Hrvatska, 11.09.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
proteomics ; gingival tissue ; periodontitis ; azurocidin ;

Sažetak
Objectives: The aim of this study was to establish changes in protein expression between gingival tissues samples in healthy subjects (HH, control group) and healthy sites, sites with gingivitis, periodontitis, and post treatment sites in patients with generalized aggressive periodontitis (GAgP, test groups). Methods: The study enrolled 8 patients, 4 with GAgP, and 4 with no signs of periodontal disease referred for crown lengthening. Gingival tissue samples in GAgP were harvested during initial periodontal treatment and sampled as healthy (PD≤3mm, BOP-), gingivitis (PD≤3mm, BOP+), periodontitis (PD≥4mm), 3 months post treatment, and in HH during surgical procedures. Tissue samples were stored in RNAlater reagent according to the manufacturer’s instructions until further analyses. Proteins from tissue lysate were separated by 1D gel electrophoresis, in-gel digested with trypsin, and measured by nano-scale HPLC system coupled to a mass spectrometer through a nano-electrospray ionization source. Shotgun label-free quantitative proteomics was used to identify proteins. False discovery rate was set to 5%. The obtained raw data was processed by MaxQuant software. Results: The total number of identiÕed proteins was 3420. After statistical data processing, 193 proteins showed statistically signiÕcant changes in expression levels between the control group and test groups (Image 1). Proteins that could possibly be a mark of periodontal health are: Multimerin-1, Annexins, Prolargin ; of gingivitis: Dedicator of cytokinesis protein 2, Band 3 anion transport protein, Azurocidin ; of periodontitis: Azurocidin, Myeloid cell nuclear diáerentiation antigen, Histones, Immunoglobulins ; and of post treatment condition: Oligosaccharyltransferase complex subunit protein, Prolargin and Mimecan. The most interesting protein was Azurocidin, showing the highest fold change in periodontitis and gingivitis. Conclusions: Results showed diáerent protein expression in tested groups and conÕrmed that proteomic analyses of periodontal tissues could contribute to a better understanding of the pathogenesis of periodontal diseases, and might contribute to the improvements in diagnosis and prognosis of GAgP.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

POVEZANOST RADA