Naslov
Validation of quantitative chromatographic analysis on laboratory-prepared thin layers

Autori
Petrović, Mira ; Kaštelan-Macan, Marija

Izvornik
Journal of chromatography. A (0021-9673) 704 (1995); 173-178

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
amino acids ; laboratory-prepared plates ; validation

Sažetak
Validation of the determination of amino acids on laboratory-prepared plates was performed on a mixed natural zeolite and microcrystalline cellulose sorbent. Chromatograms were recorded in the visible region using a Datacolor DC 3890 reflectance spectrometer, Camag Turner Fluorimeter 111 slit- scanning densitometer and Leco 2001 image analyser. The precision, detection limit and limit of quantification for each method were determined. A lower total R.S.D. was obtained by measuring colour remission with the Datacolor system (2.7% for high concentration and 3% for low concentration), with an instrumental R.S.D. of 0.1-0.2%. Densitometry and image analysis resulted in a lower precision, especially at low concentration (total R.S.D.>10%), with a significant instrumental error.

Izvorni jezik
Engleski

POVEZANOST RADA