Naslov
Chemically functionalized single-walled carbon nanotubes alter the cytokine profile of the stretch-injured cultured mouse astrocytes
(Chemically functionalized single-walled carbon nanotubes alter the cytokine profile of the stretch-injured cultured mouse astrocytes)

Autori
Gržeta, Nika ; Harej Hrkać, Anja ; Parpura, Vladimir ; Župan, Gordana ; Pilipović, Kristina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Booklet / - , 2021, E480-E480

Skup
15th European Meeting on Glial Cells in Health and Disease

Mjesto i datum
Online, 05.07.2021. - 09.07.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
astrocytes ; carbon nanotubes ; cytokines ; traumatic brain injury

Sažetak
Traumatic brain injury (TBI) is one of the leading causes of death and disability worldwide and represents a significant public health problem. In the central nervous system, astrocytes act as regulators of the brain environment and have a role in the synaptic plasticity and the reorganization of neural circuits following TBI. Carbon nanotubes represent promising nano candidates in treating brain diseases and injuries. They have excellent physicochemical properties, and the ability to interact with neurons, neuronal circuits, and astrocytes. The aim of our study was to test the effects of the application of chemically functionalized single-walled carbon nanotubes (SWCNTs) on the release of cytokines from the primary mouse astrocytes exposed to severe in vitro TBI. All research was performed on the primary astrocyte cultures that were grown on 6-well plates with silastic membranes. Severe stretch- injury was induced by controlled release of pressurized nitrogen, with a peak pressure of 3.8-4.2 PSI. One-hour post-stretch, SWCNTs, or vehicle were applied and 23 h later cell media were collected for the determination of the cytokine profile. Non-injured, vehicle-treated astrocytes were used as the control group. For the measurement of the cytokine levels, we utilized the Mouse Cytokine Array C3 kit (RayBiotech, Inc.), which can detect changes in expression of 62 different proteins with high sensitivity (pg/ml). Application of the chemically-functionalized SWCNTs to the injured cells caused an increase in the release of the axl, BLC, GM-CSF, IL3 Rb, IL4, IL5, IL6, and IL10 in regards to the levels determined in the cell media from the non-injured cells. Astrocytes treated with the investigated nanomaterial also released higher amounts of axl, BLC, G-CSF, GM-CSF, IL4, IL5, IL6, IL9, and IL10 compared to the levels determined in the samples from the injured, vehicle-treated cells. Our results showed that the investigated SWCNTs cause changes in the cytokine release from the cultured astrocytes exposed to severe in vitro TBI pointing to the possible mechanism of their effects in the injured neural tissue. Acknowledgment This research was fully supported by the Croatian Science Foundation grant UIP-2017-05- 9517 to KP.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA