Naslov
Optimization of MAE for the Separation of Nicotine and Phenolics from Tobacco Waste by Using the Response Surface Methodology Approach

Autori
Banožić, Marija ; Banjari, Ines ; Flanjak, Ivana ; Paštar, Mate ; Vladić, Jelena ; Jokić, Stela

Izvornik
Molecules (1420-3049) 26 (2021), 14; 4363, 14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
tobacco waste ; microwave-assisted extraction ; optimization ; bioactive compounds

Sažetak
This study intends to valorize by-products of the industrial processing of tobacco to obtain nicotine and phenolics as value-added compounds. Three influential parameters of the microwaveassisted extraction-MAE (temperature, treatment time, and solvent/solid ratio) were studied for the optimization of the extraction protocol for tobacco leaves and three types of waste—scrap, dust, and midrib, respectively. Nicotine was the dominant bioactive compound in all extracts, ranging from 1.512 to 5.480% in leaves, 1.886 to 3.709% in scrap, 2.628 to 4.840% dust, and 0.867 to 1.783% in midrib extracts. Five phenolic compounds were identified and quantified, predominated by chlorogenic acid and rutin. Additionally, total phenol content and antioxidant activity were determined using spectrophotometric assays. Optimization was performed in two aspects: to obtain a maximum extraction yield with minimum nicotine content and to obtain a maximum extraction yield with maximum nicotine content. These findings demonstrate that tobacco waste is a valuable source of bioactive compounds and MAE can be a promising alternative technique to obtain extracts rich in targeted bioactive compounds, especially nicotine.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Farmacija, Biotehnologija, Prehrambena tehnologija, Interdisciplinarne biotehničke znanosti

Napomena
This article belongs to the Special Issue
Ultrasound- and Microwave-Assisted Extraction of
Bioactive Compounds

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