Naslov
Streptozotocin-induced diabetes causes age-dependent increase in sensitivity of ca1 neurons to aglycemia

Autori
Pađen, A.L ; Tekkök, S ; Križ, J ; Krnjević, K.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Society for Neuroscience : Abstracts / Society for Neuroscience - Washington (MD) : Society for Neuroscience, 1997, 349-349

Skup
27th Annual Meeting, Society for Neuroscience

Mjesto i datum
New Orleans (LA), Sjedinjene Američke Države, 25.10.1997. - 30.10.1997

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
STR-diabetes; CA1 neurons; hippocampus; aglycemia

Sažetak
We have investigated synaptic function of hippocampal neurons during different forms of aglycemia. Hippocampal slices were cut from the brains of streptozotocin (STZ)-treated male Wistar rats at 6 (young) and 12 month (older), as well as from age-matched control rats, and kept submerged in ACSF at 33oC. Field EPSPs and population spikes, evoked by stimulation of st. radiatum, were recorded simultaneously from st. radiatum and pyramidale. Aglycemia was induced by substituting ACSF glucose with 10 mM sucorse, 5 mM 3-hydrixybutyric acid (3OHBA) and 5 mM mM sucorse, or 10 mM 2-deoxy-D-glucose (2-DG). Sucrose (10 mM) blocked transmission much more rapidly in slices from young diabetic rats (14ą0.5 min) than in control slices (22.3ą13 min n=3-5). Similarly, when 10 mM glucose in ASCF was replaced by 5 mM 3OHBA and 5 mM sucrose, transmission was blocked in 18ą0.9 min in diabetic slices and 37ą1.1 min in controls (n=3). By contrast, blocking times in 2-DG did not differ significantly in young diabetic (15ą1.1 min) and control slices (13ą0.53 min, n= 3-15). In slices from older diabetic rats, times for transmission block by 10 mM sucrose did not differ between diabetic and control groups (15ą0.4 vs 12ą0.5 min, n=4-6). Synaptic block times in 2-DG were also similar in both groups (12ą0.3 min vs 12ą0.5 min, n=6-9). In conclusion, in slices from young STZ-induced diabetic rats, synaptic tranmission is more sensitive to lack of glucose and is not better preserved by ketone body supplement (3OHBA). This presumably reflects adaptation to higher glucose levels in CNS of diab etic rats, which may be impaired by aging.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA