Naslov
Degradation Products of Azetidine Core G334089 - Isolation, Structure Elucidation and Pathway

Autori
Čikoš, Ana ; Dragojević, Snježana ; Kubiček, Adrijana

Izvornik
Journal of pharmaceutical and biomedical analysis (0731-7085) 203 (2021); 114232, 7

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
degradation products ; azetidine ; GLPG0974 ; FFA2 ; LC-MS ; NMR ; esterification

Sažetak
An extensive forced degradation study using hydrolytic degradation conditions was performed on G334089, the S-enantiomer of the free fatty acid receptor 2 (FFA2) antagonist GLPG0974, to identify the degradation product structures and discern degradation pathways. Not all degradation products gener- ated ions in the MS spectra, while several others were isomers, so more rigorous degradation conditions were applied to increase the degradant yield. Esterification of the degradants facilitated isolation via preparative HPLC and subsequent NMR and MS characterisation. The determined structures, retention times and fragmentation patterns were used to identify the original degradation products and postu- late a degradation pathway. In addition to the expected amide bond hydrolysis, a second degradation mechanism involving azetidine activation through formation of an azetidinium ion was demonstrated.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA