Naslov
Spectrophotometric Folin-Ciocalteu and Aluminum chloride method validation for determination of phenolic acid, flavan-3-ol, flavonol and anthocyanin content

Autori
Matić, Petra ; Jakobek, Lidija

Izvornik
Croatian journal of food science and technology (1847-3466) 13 (2021), 2; 176-183

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
total polyphenols ; total flavonoids ; validation

Sažetak
Spectrophotometric methods for the determination of total polyphenols and total flavonoids are often used because they are simple, sensitive and precise. Before using these methods, they must be validated to ensure the quality of the obtained data and to prove that the method is suitable for its purpose. The aim of this work was to validate the spectrophotometric methods for total polyphenols and total flavonoids. The method for total polyphenols has been validated according to polyphenol standards which belong to the groups of phenolic acids (p-hydroxybenzoic acid, sinapic acid, ferulic acid), flavan-3-ols ((-)-epicatechin), flavonols (myricetin, kaempferol) and anthocyanins (cyanidin-3-glucoside). For the validation of the method for total flavonoids, the same polyphenol standards belonging to flavan-3-ols, flavonols and anthocyanins have been used. The validation parameters were linearity, sensitivity, limit of detection, limit of quantification, precision and accuracy. The results of the validation for both methods were in acceptable ranges, except for kaempferol in the method for total flavonoids. In conclusion, methods can be used for the determination of total polyphenols and total flavonoids by using calibration curves of studied polyphenol standards, except for the standard kaempferol in the method for total flavonoids due to lower sensitivity.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA