Naslov
Detection of melanoma cells in peripheral blood by reverse transcription - poymerase chain reaction

Autori
Šamija, Ivan ; Hrašćan, Reno ; Jazvić, Marijana ; Kusić, Zvonko ; Lukač, Josip ; Marić-Brozić, Jasmina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts. 1st Croatian Congress on Molecular Life Sciences / Dumić, Jerka - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2002, 174-174

ISBN
953-6256-13-4

Skup
1st Croatian Congress on Molecular Life Sciences

Mjesto i datum
Opatija, Hrvatska, 09.06.2002. - 13.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
tyrosinase; RT-PCR; circulating tumor cells; melanoma

Sažetak
Reverse trasnscription – polymerase chain reaction (RT-PCR) for tyrosinase mRNA is specific and sensitive method for detection of circulating melanoma cells in peripheral blood of melanoma patients. Most of the studies have shown that detection of tyrosinase mRNA by RT-PCR in peripheral blood of melanoma patients correlates with clinical stage and disease prognosis. Still, clinical value of this method is under debate. Sensitivity of the method we used was high enough to detect single HBL melanoma cell on 0.82x106 peripheral blood leukocytes. In this study 293 blood samples from 148 melanoma patients in all clinical stages of the disease were analysed. From 75 of these patients samples were obtained more than once at one month or longer time intervals. Circulating melanoma cells were detected in 7.5 % of 161 blood samples from patients with localised disease (AJCC stages I and II), in 8.1 % of 98 blood samples from patients with regional lymph node metastases (AJCC stage III) and in 26.5 % of 34 blood samples from patients with distant metastases (AJCC stage IV). The positivity rate for individual patients was higher if only one positive test was required to label a patient tested more than once positive: 13.3 % for 98 patients with AJCC stage I and stage II disease, 12 % for 50 patients with AJCC stage III disease, and 42 % for 19 patients with AJCC stage IV disease. Tyrosinase mRNA was not detected in control samples from healthy volunteers. In our study we confirmed high sensitivity and specificity of RT-PCR for tyrosinase in detection of circulating melanoma cells in peripheral blood of melanoma patients. Although the overall percentage of positive samples was low (9.9 % of 293 samples), the detection of tyrosinase mRNA was significantly more frequent in patients with distant metastases, than in patients with local disease or with local lymph node metastases. Also, our results show that the presence of tyrosinase-positive cells in peripheral blood as detected by this method is a discontinuous phenomenon.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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