Naslov
Potential gain of genome editing for improved animal breeding

Autori
Nikola, Raguž ; Boris, Lukić

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
55. Hrvatski i 15. Međunarodni Simpozij Agronoma : Zbornik radova = 55th Croatian & 15th international symposium on agriculture : book of abstracts / Mioč, Boro ; Širić, Ivan - Zagreb : Agronomski fakultet Sveučilišta u Zagrebu, 2020, 464-468

Skup
55. hrvatski i 15. međunarodni simpozij agronoma

Mjesto i datum
Vodice, Hrvatska, 16.02.2020. - 21.02.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
genetic variation ; genome editing ; animal breeding ; CRISPR ; Cas9

Sažetak
Genetic variability is the main precondition that enables both natural and artificial selection. It occurs through spontaneous mutations, recombination processes during meiosis, gamete combinations during fertilization or is induced by mutagenesis (Kawall, 2019). Mutations are the main tool of natural selection and represent any change in genetic material that is not a consequence of two individuals crossing. Genome editing is also related to DNA sequence change by deletions, mRNA processing and post-transcriptional modifications that results in altered gene expression and functional behaviour of proteins (Khan, 2019). It is based on highly specific and programmable nucleases, which produce specific changes in regions of interest in the genome by introducing double-strand breaks (DSBs) that are later repaired by cellular mechanisms. Repair permits the genesis of insertions, deletions or substitutions in the target area. These mutations may prevent, eliminate or correct the defects in genes (RodriguezRodriguez et al., 2019). The recent expansion and advancements in the field of biotechnology, especially in animal breeding, have provided more information and insight into the biochemical and molecular mechanisms to edit DNA. The new techniques seem promising, but the earlier ones have also been updated and improved. For simplicity and consolidation, an overview of genome editing techniques as well as their potential use in animal breeding will be presented below.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija

POVEZANOST RADA