Naslov
Association between human papillomavirus and EGFR mutations in non-small cell lung cancer

Autori
Harabajsa, Suzana ; Šefčić, Hajdi ; Šimić, Vesna ; Vrabec Branica, Božica ; Klasić, Marija ; Milavić, Marija ; Židovec Lepej, Snježana ; Grgić, Ivana ; Zajc Petranović, Matea ; Badovinac, Sonja ; Jakopović, Marko ; Smojver-Ježek, Silvana ; Korać, Petra

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
Toraks 2021 : 11. kongres Hrvatskog torakalnog društva

Mjesto i datum
Online, 19.05.2021. - 22.05.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
HPV ; EGFR ; NSCLC ; meta-analysis

Sažetak
Aim: High-risk types 16 and 18 of human papillomavirus (HPV) are associated with the development and progression of various cancers. Unlike classical, the presence of rare mutations in epidermal growth factor receptor (EGFR) gene in non-small cell lung cancer (NSCLC) is responsible for incomplete tyrosine kinase inhibitors response. The aim of this study was to determine the prevalence of HPV infection in NSCLC cytological smears in relation to the presence and frequency of EGFR gene mutations. Methods: The two-year retrospective analysis included 67 NSCLC cytological smears obtained from lung cancer patients hospitalized at the Department of Respiratory Diseases Jordanovac, University Hospital Centre Zagreb. Cytological samples were routinely processed to obtain good quality smears with a sufficient number of malignant cells for PCR analysis. The DNA was extracted from NSCLC cytological smears stained by MGG using the Cobas DNA Sample Preparation Kit. Analyzed EGFR gene mutations were divided into classical (ex19del ; L858R) and rare (G719X ; ex20ins ; S768I ; T790M and ex21). The presence of HPV16 and/or HPV18 DNA was detected by amplifying the fragment of the E7 gene. Expected PCR product size was 212 bp for HPV16, and 236 bp for HPV18. Prevalence of HPV infection in NSCLC patients with EGFR mutations compared to NSCLC patients without EGFR mutations was additionally examined by meta- analysis using data from this and six other studies. Results: Among the 67 NSCLC cytological smears, 34 had mutations in the EGFR gene and 33 were free of it. More frequent HPV infection was observed in the NSCLC smears with EGFR gene mutations (HPV16, n=11/34, p<0.05 ; HPV18, n=24/34, p<0.001) compared to the NSCLC smears without EGFR gene mutations. Significant association between smoking and HPV16 infection was observed (p<0.028) in the group of NSCLC patients with EGFR gene mutations. Among 34 NSCLC cytological smears, 46 EGFR gene mutations were detected. Classical EGFR gene mutations were detected in 20 (58.8%) NSCLC smears, while rare mutations in five smears (14.7%). Nine (26.5%) NSCLC cytological smears had a combination of classical and rare EGFR gene mutations. The significant association between the presence of rare EGFR gene mutations and HPV16 and/or HPV18 infection (p<0.05) was observed. Meta-analysis confirmed that NSCLC patients with EGFR gene mutations have higher odds to have HPV infection than those without mutations. Conclusion: The higher prevalence of HPV infection suggests the role of this virus in the etiology of NSCLC with EGFR gene mutations.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kliničke medicinske znanosti

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