Naslov
Could Samples Stored at Room Temperature for up to 24 Hours Be Accepted for Second-Line Coagulation Testing?

Autori
Rimac, Vladimira ; Coen Herak, Desiree

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Clinical chemistry and laboratory medicine / Plebani, Mario - , 2015, EA8-eA9

Skup
3rd EFLM-BD European Conference on Preanalytical Phase

Mjesto i datum
Porto, Portugal, 20.03.2015. - 21.03.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
routine coagulation tests, 24-h stability, room temperature

Sažetak
Background: Accurate coagulation test results depend on all phases of laboratory process. Besides sampling, transport and centrifugation, sample stability following blood collection is an important part of preanalytical phase, but it is still a matter of concern. Although samples for coagulation testing are routinely processed according to current guidelines, sometimes there are requests for performing additional tests in already analyzed fresh plasma samples, especially in critical care patients. The aim of this study was to examine whether fresh plasma samples stored at room temperature for up to 24-h after blood collection can still be accepted for second- line routine coagulation testing. Materials and methods: We have measured prothrombin time (PT), activated partial tromboplastin time (aPTT), fibrinogen (Fbg) and antithrombin (AT) in citrated plasma samples in primary tubes within 4-h of blood collection and after storage at 25 °C for up to 24-h. Additionally, plasma aliquots were separated from primary tubes and stored at 25 °C for up to 24-h. Stored plasma samples in primary tubes and aliquots were processed the next day under the same conditions. For each parameter, samples from 20–30 patients were investigated covering a wide range of normal and pathological results. Measurements were performed on the coagulation analyzer BCS XP by using reagents from Siemens Healthcare Diagnostics. Results: The highest variation of results in stored samples was observed for aPTT (primary tubes: mean 11.8%, range 3.4–32.8% ; aliquots: mean 15.5%, range 5.0–30.4%). For all other parameters the mean percentage change was below 10% either in primary tubes (ranging from 1.3– 5.7%) or in aliquots (ranging from 1.1–3.6%). Conclusion: No advantage of separating plasma from cells was observed. Samples stored in primary tubes for up to 24-h can be used for second-line routine coagulation testing, even for aPTT as changes of results were not clinically relevant.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

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