Naslov
Mass spectrometry-back-to-basic
(Mass spectrometry-back-to-basics)

Autori
Cindrić, Mario

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The 12th Greta Pifat Mrzljak International School of BiophysicsBiomolecular complexes and assemblies / Book of AbstractsPrimošten, Croatia / 27 September – 6 October 2014 / Vuletić, Tomislav ; Hozić, Amela - Zagreb : Institut Ruđer Bošković ; Hrvatsko biofizičko društvo, 2014, 20-20

ISBN
978-953-7941-02-4

Skup
The 12th Greta Pifat Mrzljak International School of Biophysics

Mjesto i datum
Primošten, Hrvatska, 27.09.2014. - 06.10.2014

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Mass spectrometry, ionization, basics

Sažetak
The basics of mass spectrometry will be considered, starting with instrumentation, followed by different techniques of ionization and fragmentation necessary to understand development of mass spectrometry during the past two decades. Particularly, this tutorial lecture will be focused on technical aspects of mass spectrometry:-ion sources-ion optics-ion separation-cells for fragmentation-ion detectors-collecting MS data-data processingTechniques for molecules and macromolecules separation and fractionation hyphenated to mass spectrometers, particularly mass spectrometry techniques for the large scale identification of proteins will be reviewed. Also, different methods for protein quantification by mass spectrometry will be described and discussed. Finally, bioinformatics principles to convert mass spectrometry data into peptide/protein sequences and to quantify them will be introduced.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA