Naslov
Early Childhood Fat Tissue Changes—Adipocyte Morphometry, Collagen Deposition, and Expression of CD163+ Cells in Subcutaneous and Visceral Adipose Tissue of Male Children

Autori
Mujkić, Robert ; Šnajder Mujkić, Darija ; Ilić, Ivana ; Rođak, Edi ; Šumanovac, Antun ; Grgić, Anđela ; Divković, Dalibor ; Selthofer-Relatić, Kristina

Izvornik
International journal of environmental research and public health (1661-7827) 18 (2021), 7; 3627, 10

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
obesity, early years, collagen, inflammation, macrophages, adipose tissue

Sažetak
Abstract: Childhood obesity is a complex health problem, and not many studies have been done on adipose tissue remodeling in early childhood. The aim of this study was to examine extracellular matrix remodeling in the adipose tissue of healthy male children depending on their weight status. Subcutaneous and visceral adipose tissue was obtained from 45 otherwise healthy male children who underwent elective surgery for hernia repairs or orchidopexy. The children were divided into overweight/obese (n = 17) or normal weight groups (n = 28) depending on their body mass index (BMI) z- score. Serum was obtained for glucose, testosterone, triglyceride, total cholesterol, high-density lipoprotein (HDL), and low-density lipoprotein (LDL) measurements. Sections of adipose tissue were stained with hematoxylin and eosin to determine the adipocytes’ surface area, and Masson’s trichrome stain was used to detect the adipocytes’ collagen content. Immunohistochemistry for CD163+ cells was also performed. The results showed that male children in the overweight group had higher serum triglyceride levels, greater adipocyte surface area and collagen content in their subcutaneous adipose tissue, more crown-like structures in fat tissues, and more CD163+ cells in their visceral adipose tissue than males in the normal weight group. In conclusion, in male children, obesity can lead to the hypertrophy of adipocytes, increased collagen deposition in subcutaneous adipose tissues, and changes in the polarization and accumulation of macrophages.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

Napomena
This research was partly funded by the internal
research grant from the Faculty of Medicine
Josip Juraj Strossmayer, University of Osijek
[grant number VIF2017-MEFOS-9] and Faculty of
Dental Medicine and Health Josip Juraj
Strossmayer, University of Osijek [grant number
VIF-2018-FDMZOS-05]. The APC was funded by
Faculty of Dental Medicine and Health Science
Fund.

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