Pregled bibliografske jedinice broj: 1121934
ADHESION AND AGGREGATION CAPACITY AS IMPORTANT VIRULENCE TRAINS OF UROPATHOGENIC ENTEROCOCUS FAECALIS/FAECIUM STRAINS
ADHESION AND AGGREGATION CAPACITY AS IMPORTANT VIRULENCE TRAINS OF UROPATHOGENIC ENTEROCOCUS FAECALIS/FAECIUM STRAINS // FEMS Online Conference on Microbiology Electronic Abstract Book
Beograd, Srbija; online, 2020. str. 292-292 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1121934 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
ADHESION AND AGGREGATION CAPACITY AS IMPORTANT
VIRULENCE TRAINS
OF UROPATHOGENIC ENTEROCOCUS FAECALIS/FAECIUM
STRAINS
Autori
Repac Antić, Davorka ; Begić, Gabrijela ; Gobin, Ivana ; Abram, Maja
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
FEMS Online Conference on Microbiology Electronic Abstract Book
/ - , 2020, 292-292
Skup
FEMS Online Conference on Microbiology
Mjesto i datum
Beograd, Srbija; online, 28.10.2020. - 31.10.2020
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Enterococcus, Adhesion, Aggregation
Sažetak
Enterococci, which are part of enteric microbiota, are among the most commonly isolated gram-positive bacteria in the clinical microbiology laboratory. Bacterial adhesion and subsequent colonization in host tissues represent important virulence factors crucial for early critical step of the pathogenesis. Therefore, we examined the auto-aggregation and adhesion capacity of Enterococcus strains isolated from urine. Fifty E. faecalis and 20 E. faecium isolates were included in this study. They were collected in urine, from patients admitted to the Clinical Hospital Center Rijeka. The auto aggregation ability of these bacteria, as well as the adhesion to HeLa cells was tested. Bacterial suspension in RPMI with 10% of FBS (~106 bacteria/ml) was added to adherent HeLa cells (MOI 10:1). After 30 min incubation, number of adherent cells was determined by plate count. For aggregation, bacterial suspension was made in sterile PBS (~10(9) bacteria/ml) and absorbance was measured immediately and after and 24 h incubation at RT. Our results showed a pronounced aggregation ability of urinary E. faecalis isolates (from 92-98%). Also, uropathogenic strains were highly adherent to HeLa cells. These observations implied that uropathogenic E. faecalis isolates exhibit specific properties and that this is pronounced in adhesion and aggregation properties.
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
POVEZANOST RADA
Ustanove:
Medicinski fakultet, Rijeka
