Naslov
Introducing a new tissue banking activity - processing of cadaveric skin

Autori
Dolenec, Tamara ; Bujic Mihica, Marina ; Vrgoc Zimic, Ivana ; Batarilo, Ivanka ; Ulamec, Monika ; Rac, Danijela ; Puljiz, Zvonimir ; Rukavina, Lidija ; Demirovic, Alma ; Tominac Trcin, Mirna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book - 28th Congress of European Associoation of Tissue and Cell Banks / - , 2019, 70-71

Skup
28th Congress of European Association of Tissue and Cell Banks

Mjesto i datum
Liblice, Češka Republika, 16.10.2019. - 18.10.2019

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
extensive burns ; explantation and processing of cadaveric skin ; glycerol preserved allografts

Sažetak
In July 2014 production facility in our Tissue Bank at Department of Traumatology, University Hospital Centre Sestre milosrdnice, was officially licensed by Croatian Competent Authority (CA) for the production of cultured autologous keratinocytes for treatment of burns under the hospital exemption rule. Early coverage of the wound bed in burned patients is mandatory for the treatment. Glycerol preserved allografts (GPAs) are often used for this purpose, especially in extensive burns. Since 2016 we are importing GPAs from Banc de Sang i Teixits, Barcelona. Our clinical experience is that GPAs create a good environment for subsequent application of cultured autologous keratinocytes. In 2017 we decided to make a small pilot study in our University Hospital Centre involving explantation and processing of cadaveric skin. After the Hospital Ethics Committee approval and permission from our CA we have explanted cadaver skin from one Donor after Brain Death (DBD) and the two Donors after Cardiac Death (DCD). DBD and DCD donors were from 35 to 51 years old. Average cooling time of the bodies before the commencement of explantation was less than 24 hours. Thorough evaluation of a donors' social and medical history as well as blood tests were performed. Explantations were carried out in our hospital in operating theater and in autopsy room, depending on the type of the donor. Explantation team consisted of one plastic surgeon and three medical technicians. Immediately after the excision, the skin was put in solution of 50% of sterile glycerol with antibiotics and transported to the tissue bank for further processing. The largest skin quantity obtained from one donor was 1000 cm2. Skin grafts were processed in clean room, in class A with class B background and finally preserved in solution of 85% of glycerol at +4°C. Skin bioptates were taken for additional microbiological control at regular intervals up to one year after the explantation. GPAs processed in this pilot project served only for the educational and validation purposes. Additional efforts must be made in order to further educate and practice explantation team in aseptic techniques and in obtaining larger area of the skin with more uniform size.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Interdisciplinarne biotehničke znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA