Naslov
The actin-modulating protein synaptopodin mediates long-term survival of dendritic spines

Autori
Yap, Kenrick ; Drakew, Alexander ; Smilović, Dinko ; Rietsche, Michael ; Paul, Mandy H ; Vuksic, Mario ; Del Turco, Domenico ; Deller, Thomas

Izvornik
ELife (2050-084X) 9 (2020); E62944, 30

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
entorhinal denervation ; granule cell ; hippocampus ; mouse ; neuroscience ; spine apparatus ; synaptic plasticity.

Sažetak
Large spines are stable and important for memory trace formation. The majority of large spines also contains synaptopodin (SP), an actin-modulating and plasticity-related protein. Since SP stabilizes F-actin, we speculated that the presence of SP within large spines could explain their long lifetime. Indeed, using 2-photon time- lapse imaging of SP-transgenic granule cells in mouse organotypic tissue cultures we found that spines containing SP survived considerably longer than spines of equal size without SP. Of note, SP- positive (SP+) spines that underwent pruning first lost SP before disappearing. Whereas the survival time courses of SP+ spines followed conditional two-stage decay functions, SP-negative (SP-) spines and all spines of SP- deficient animals showed single-phase exponential decays. This was also the case following afferent denervation. These results implicate SP as a major regulator of long-term spine stability: SP clusters stabilize spines, and the presence of SP indicates spines of high stability.

Izvorni jezik
Engleski

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