%PDF-1.7
%
2 0 obj
<<
/Metadata 4 0 R
/Names 5 0 R
/Outlines 6 0 R
/Pages 7 0 R
/Type /Catalog
>>
endobj
4 0 obj
<<
/Length 5808
/Subtype /XML
/Type /Metadata
>>
stream
Tihomir Kovač
Tihana Marček
Bojan Šarkanj
Ivana Borišev
Maja Ižaković
Katarina Jukić
Ante Lončarić
Tamara Krska
Michael Sulyok and Rudolf Krska
fullerol C60(OH)24 nanoparticles
drought
wheat (Triticum aestivum L.)
mycotoxins
Aspergillus flavus NRRL 3251
Fullerol C60(OH)24 nanoparticles (FNP)-wheat-A. flavus interaction outcome is more complicated in the presence of drought. This study sheds light on how the presence of FNP affects food and feed safety from the perspective of mycotoxin contamination. The study aims to determine the influence of FNP at environmentally plausible concentrations on wheat growth under drought stress and on the aggressiveness of A. flavus during wheat germination, as well as the influence of FNP on the secondary metabolite profile during the inappropriate wheat storage. The co-occurrence of drought and FNP inhibited germination and shoot growth, while an application of FNP alone had no negative effect on plant growth. Wheat pre-treated with FNP showed a concentration dependent resistance pattern to A. flavus aggressiveness. Nevertheless, using a LC-MS/MS based multi-mycotoxin method, six secondary fungal metabolites: 3-nitropropionic acid (<LOD -775.7336 10.7752 ng mL-1), aflatoxin B1 (<LOD -6.78 0.43 ng mL-1) and B2 (<LOD -0.07 0.00 ng mL-1), aflatoxicol (<LOD -0.37 0.16 ng mL-1), kojic acid (<LOD -1337.87 189.04 ng mL-1), and O-methylsterigmatocystin (<LOD -0.17 0.00 ng mL-1), were detected. FNP affected secondary metabolism of A. flavus during inappropriate wheat storage and increased the concentration of secondary metabolites in a concentration-dependent pattern (3-nitropropionic acid and kojic acid). In addition, aflatoxicol production was provoked in FNP treated samples.
Fullerol C60(OH)24 Nanoparticles and Drought Impact on Wheat (Triticum aestivum L.) during Growth and Infection with Aspergillus flavus
application/pdf
2021-03-22T07:51:48+01:00
LaTeX with hyperref
2021-03-22T11:51:09+01:00
fullerol C60(OH)24 nanoparticles; drought; wheat (Triticum aestivum L.); mycotoxins; Aspergillus flavus NRRL 3251
pdfTeX-1.40.21
uuid:3e8d2094-e024-4d33-9881-8b1a43f2789c
uuid:44e15761-488e-46dd-b50b-a02a03ea22ea
endstream
endobj
54 0 obj
<<
/Filter [/FlateDecode]
/Length 5645
>>
stream
xڵxFg\ʹ -vDܼn+?=x~Wճ¿E$1a* 'ߞ I=ܹ <8_^d4ݚd0-~;:*"`Ϟ0%bjy5(AgSS6b~tF5I9Kp<`lN ׇ6ٜ8fqXbf%Dhnx>2=~ep% MECﳲgv%?+U]֛J(XVB&8$ۓC2L $3Yʢ ;GB
\an[bb-uQuunFK,W-`m#3`z >h5<_bP c(*eTl]Qol. bOhуDjj@iȜ:Hdssepp?AKκ(C͔D?'C cցَV@hGYܹcsVf19d#6? e\Oa c$$^V_3g_ \V_gIQʐ?,PC4 *N$M9uy)˪.0)NhCV)AtܫWKLuv]V87a}PprSH#20H)-RxG%Z$ v9>{ r~$`ΏRevb|S7ىR54obr|:liV7F^4k~@pr(.xXVRH8IIȩ4OUO[̹9l$X*Gbw; $d_@:r `d_HI`
D<.6ì;/>
sD>יtB2g\>֙t@}P.,d Ƅdt"?O9/c)p# MDl.dt0mpoU[/d^O(GQKu#RʀB,k!\aaQbҪ1Keƾ7c3s-.x<͵5\Ĝ*L"JL{u`+n<>xi~쎈Z*:w $f^sPcݞ[VWd 9xZ{VtMLҊhtūHzM0(1ӬWQB1*)Y°VGHk)vh$R"8ЀNI+"z)b!\}4I`?ōf1SXi"4ROFp˛1WT
aڔŽ/shӶϗUA*xNcy?.w(J<pCh(M
8FO\"[baeVw $GB3mQ:/߃rcȋ|3*