Naslov
Callus induction and plant regeneration from immature and mature embryos and immature inflorescences of eight Croatian winter wheat cultivars (Triticum aestivum L.)

Autori
Kereša, Snježana ; Barić, Marijana ; Šarčević, Hrvoje ; Marchetti, Stefano

Izvornik
Die Bodenkultur (0006-5471) 54 (2004), 3; 155-161

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
winter wheat; embryo culture; immature inflorescences; plant regeneration

Sažetak
Eight Croatian winter wheat cultivars (Triticum aestivum L.) were evaluated for their ability to produce callus and to regenerate whole plants from immature and mature embryos and immature inflorescences. The highest mean percentage regeneration across cultivars (21.6%) was obtained when immature embryos were used as explant with the highest regeneration of cultivar Žitarka (57%) and Edita (54%). However, some cultivars regenerated equally efficiently (Edita) or better (Lipa) when immature inflorescences were used. The highest regeneration capacity from mature embryos (26% for cultivar Magdalen) was much below the best regeneration capacities from the immature embryo or inflorescence culture. As far as the media provided with 2, 4-D were used for callus induction from immature embryos, plant regeneration was significantly better than in a medium with picloram. With immature inflorescences, on the contrary, the best culture response was obtained using medium containing picloram.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija

POVEZANOST RADA