Naslov
Agrobacterium-mediated transformation of flax (Linum usitatissimum L. cv. Sara) with the phaC1 gene

Autori
Talajić, Antea

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prehrambeno-biotehnološki fakultet

Mjesto
Zagreb

Datum
05.06

Godina
2020

Stranica
56

Mentor
Štafa, Anamarija

Ključne riječi
mcl-PHA, biopolymers, phaC1 gene, flax, Agrobacterium-mediated transformation

Sažetak
Medium-chain-length polyhydroxyalkanoates (mcl- PHAs) belong to the group of polymeric materials naturally synthesized by many species of microorganisms. These biopolymers attract increasing attention due to their biodegradability and great thermo-mechanical properties, making them an excellent alternative for petroleum-based plastics and subsequently reducing the consumption and demand for plastic- goods. Therefore, such application of mcl-PHAs would, without a doubt, make a great positive impact on the environment. However, higher production costs of microbial production of these biopolymers compared to chemical production of conventional plastics, pose a great challenge. Thus, genetically modified plants are emerging as a great platform for large-scale PHA synthesis. In this thesis, flax plants (Linum usitatissimum L. cv. Sara) were genetically transformed with the phaC1 gene, fused to the isocitrate lyase peroxisomal targeting sequence (ICL). The main objective of such genetic modification is improving mechanical properties of flax fibers for their further applications. The results indicate that flax is suitable for transformation via A. tumefaciens with the phaC1 gene construct. It was determined that transgene was successfully integrated and expressed in transformed plant lines.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA