Pregled bibliografske jedinice broj: 1110780
Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells
Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells // Single Domain Antibodies / Saerens, Dirk ; Muyldermans, Serge (ur.).
Totowa (NJ): Humana Press, 2012. str. 287-303 doi:10.1007/978-1-61779-968-6_18
CROSBI ID: 1110780 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Stable Expression of Chimeric Heavy Chain
Antibodies in CHO Cells
Autori
Agrawal, Vishal ; Slivac, Igor ; Perret, Sylvie ; Bisson, Louis ; St-Laurent, Gilles ; Murad, Yanal ; Zhang, Jianbing ; Durocher, Yves
Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, ostalo
Knjiga
Single Domain Antibodies
Urednik/ci
Saerens, Dirk ; Muyldermans, Serge
Izdavač
Humana Press
Grad
Totowa (NJ)
Godina
2012
Raspon stranica
287-303
ISBN
978-1-61779-967-9
Ključne riječi
Fc-fusions ; PEI-max ; Antibody-production ; Cell-line-development ; Antibiotic-selection
Sažetak
Camelid single domain antibodies fused to noncamelid Fc regions, also called chimeric heavy chain antibodies (cHCAb), offer great potential as therapeutic and diagnostic candidates due to their relatively small size (80 kDa) and intact Fc. In this chapter, we describe two approaches, limiting dilution and minipools, for generating nonamplified Chinese hamster ovary cell lines stably expressing cHCAb in suspension and serum- free cultures using a stringent antibiotic selection. Neither of the protocols necessitates the acquisition or implementation of expensive automated infrastructures and thus could be applied in any lab with minimal cell culture setup. The given protocol allows the isolation of stable clones capable of generating up to 100 mg/L of antibody in batch mode performed in shaker flasks.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Biotehnologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)
