Naslov
The effect of phosphatidylcholine depletion on biochemical and physical properties of a Neurospora crassa membrane mutant

Autori
Juretić, Davor

Izvornik
Biochimica et biophysica acta. Biomembranes (0005-2736) 469 (1977), 2; 137-150

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
spin label probes, Neurospora crassa, membrane mutant, phosphatidyl choline, chol-1 choline auxotroph, choline starvation, triglycerides

Sažetak
By using the choline starvation process it is possible to deplete the membranes of Neurospora crassa choline auxotroph chol-1 of phosphatidylcholine, without affecting the viability of germinated spores or whole mycelium. Spin label probes were used to examine the possible dependence of the physical state of cellular lipids on the presence of phosphatidylcholine in the membranes. Increased freedom of rotational motion of lipid soluble probes was regularly detected in choline- starved mycelium. The accumulation of neutral lipids (mostly triglycerides) in bulk form was also observed during the choline starvation process. The experiments with isolated and separated lipid classes indicated that the observed increase in fluidity of lipids in choline-starved mycelium is partly due to the difference in physical properties between bulk lipids and membrane lipids. Spin label probe 2N4 (2-propyl-2, 5, 5-trimethyl-oxazolidine-N-oxyl), which can partition at the membrane-water interface, exhibited easier partitioning among membrane lipids of choline-starved mycelium.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija

Napomena
Automatska indeksiranost nalazi samo Scopus
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Indexing and online journal BBA is abstracted and
indexed by BIOSIS, Chemical Abstracts Service,
Current Contents/Life Sciences, EMBASE,
EMBiology, Index Chemicus, MEDLINE/Index Medicus,
Science Citation Index, and Sociedad
Iberoamericana de Informacion Cientifica

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