Naslov
Magainin 2 amide and analogues Antimicrobial activity, membrane depolarization and susceptibility to proteolysis

Autori
Juretić, Davor ; Chen, Hao-Chia ; Brown, Judith H. ; Morell, John L. ; Hendler, Richard W. ; Westerhoff, Hans V.

Izvornik
FEBS letters (0014-5793) 249 (1989), 2; 219-223

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Magainin ; Proteolysis ; Membrane potential ; Respiration ; Cytochrome oxidase liposome ; Uncoupling ; (E. coli)

Sažetak
We compared the abilities of synthetic magainin 2 amide and its analogues to inhibit the growth of Escherichia coli and to cause membrane depolarization in E. coli cells and cytochrome oxidase liposomes. The analogue, magainin A, was about 40-times more active than magainin 2 amide in inhibiting the growth of E. coli and had a much more sustained effect on the membrane potential. In the liposomal system, however, there was only approx. 20% difference between these two peptides in the reduction of membrane potential and uncoupling of respiration. Studies with pronase digestion suggested that the difference in potency may be due to differential susceptibility to proteolysis in the presence of membranes.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija

POVEZANOST RADA